Near isogenic lines (NILs) are ideal material for a variety of genetic studies including validation of specific QTL. In the present study, eight pairs of NILs for grain weight were developed, seven in the background of Raj3765, and one in the background of K9107. For this purpose, marker-assisted selection (MAS) was used for the transfer of three grain weight QTL (QGw.ccsu-1A.2, QGw.ccsu-1A.3 and QGw.ccsu-1B.1) that were earlier identified in our laboratory. Two genotypes of each of the eight pairs of NILs, differed for QTL alleles (QTL_Hgw derived from the donor parent and the QTL_Lgw derived from the recipient parent). Each pair of NILs involved a solitary QTL except one NIL, which differed for all the three QTL. The difference in thousand grain weight (TGW) in two NILs of an individual pair ranged from 2.8 to 7.5 g, thus validating the effect of the QTL for TGW, although the quantum of difference did not always match the phenotypic variance of the corresponding QTL. As expected, the NILs which involved all the three QTL had the maximum difference of 7.5 g in TGW, and the NILs which involved QTL, QGw.ccsu-1A.2 had minimum average difference of 2.8 g for TGW. The NILs produced during the present study may be used in future for MAS and for fine mapping of TGW QTL.

中文翻译：

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使用MAS衍生的成对NIL在面包小麦（Triticum aestivum L.）中对籽粒重量进行QTL验证

近等基因系（NIL）是进行各种遗传研究（包括验证特定QTL）的理想材料。在本研究中，开发了八对谷物重量的NIL，其中七对在Raj3765的背景下，另一对在K9107的背景下。为此，使用了标记辅助选择（MAS）来转移较早确定的三个谷物重量QTL（QGw.ccsu - 1A.2，QGw.ccsu - 1A.3和QGw.ccsu - 1B.1）。在我们的实验室。八对_NIL中的每一个的两个基因型，对于QTL等位基因（来自供体亲本的QTL _Hgw和QTL _Lgw派生自接收方父级）。每对NIL都涉及一个单独的QTL，除了一个NIL之外，这三个QTL均不同。一对单独的两个NIL中千粒重（TGW）的差异范围为2.8至7.5 g，从而验证了QTL对TGW的影响，尽管差异的数量并不总是与相应QTL的表型变异相匹配。不出所料，涉及所有三个QTL的NIL在TGW中的最大差异为7.5 g，而涉及QTL，QGw.ccsu - 1A.2的NIL对于TGW的最小平均差异为2.8 g。在本研究过程中产生的NIL可能会在将来用于MAS和TGW QTL的精细定位。