Some pathogenic microbial species are becoming less sensitive to antibiotics and developing resistance multiples. The use of essential oils is a serious substitute for treatment with antibiotics in infectious diseases. The present study aims to investigate the chemical composition and to evaluate the antibacterial activity of the essential oil of branches of Tetraclinis articulata (Vahl) Masters (Cupressaceae). The extraction of the essential oil was realized by hydrodistillation, and the analysis was carried out by gas chromatography coupled with mass spectroscopy (GC–MS/FID). The antibacterial test was carried out using two different methods: the disc diffusion method and the macrodilution method which aims to determine the minimum inhibitory concentration (MIC). The yield of essential oil was 0.84%. Chromatographic analysis revealed that the major constituents were α-pinene (38.75%), limonene (13.24%), bornyl acetate (8.78%) and camphor (7.68%). The results of the antibacterial activity show that the essential oil exerts a powerful effect to inhibit some strains tested. The antibacterial activity demonstrated in this study could justify the traditional uses of this plant and could contribute to value its potential in order to develop new bioactive compounds.

中文翻译：

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摩洛哥东部的Tetraclinis articulata（Vahl）Masters分支机构精油的化学成分和抑菌活性

一些病原微生物物种对抗生素的敏感性降低，并产生了耐药性倍数。在传染病中，精油的使用已严重替代了抗生素治疗。本研究旨在调查化学成分和评估四叶草（Vahl）大师（柏科）的分支机构的香精油的抗菌活性。精油的提取是通过加氢蒸馏来完成的，分析是通过气相色谱-质谱联用（GC-MS / FID）进行的。使用两种不同的方法进行了抗菌测试：圆盘扩散法和旨在确定最小抑菌浓度（MIC）的大稀释法。精油的产率为0.84％。色谱分析表明，主要成分为α-pine烯（38.75％），li烯（13.24％），乙酸冰片酯（8.78％）和樟脑（7.68％）。抗菌活性的结果表明，香精油具有强大的抑制作用，可以抑制某些菌株。这项研究中证明的抗菌活性可以证明该植物的传统用途是合理的，并且可以有助于其潜力以开发新的生物活性化合物。