Background

Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported.

Methods

A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis.

Results

The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure.

Conclusion

Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.

中文翻译：

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毒死eo分析神经母细胞瘤细胞系中溴氰菊酯的暴露

背景

溴氰菊酯是一种II类拟除虫菊酯杀虫剂，由于其对昆虫的高效功效和对哺乳动物的低毒性而被广泛使用。然而，最近，据报道在溴氰菊酯接触期间可能对健康产生不利影响。

方法

使用二维电泳和质谱进行了蛋白质组学分析，并通过蛋白质印迹法验证了蛋白质组学数据。还通过分光光度分析法测量了抗氧化酶的活性。

结果

13种差异表达的蛋白质，其中8种被上调而5种被下调，它们参与了多种细胞功能，包括蛋白质折叠，细胞骨架调控和剪接过程。溴氰菊酯的暴露也改变了抗氧化酶和乙酰​​胆碱酯酶的活性。

结论

由溴氰菊酯暴露在神经母细胞瘤细胞系中引起的差异蛋白表达可以使我们开发溴氰菊酯毒性的生物标志物，并进行进一步的研究以评估溴氰菊酯毒性的分子机制。