Abstract

Sexual dimorphism of fish morphology, physiology and behavior is diverse and complex in nature. Doublesex and mab-3 related transcription factor (Dmrt) is a large protein family whose function is sexual development and differentiation in vertebrates. Here, we report a full-length cDNA sequence of Labeo rohita (rohu) Dmrt1 of 907 bp length having 798 bp of open reading frame encoding 265 amino acids. The molecular weight of rohu DMRT1 protein was found to be 28.74 KDa and isoelectric point was 7.53. DMRT1 protein contains 23 positively and 24 negatively charged amino acids with a GRAVY score of -0.618. A characteristic DM domain was found in DMRT1 protein, which is a novel DNA-binding domain. Phylogenetic analysis showed maximum similarity with Cyprinus carpio when compared with DMRT1 of other vertebrates. Molecular docking study identified active sites to be targeted for drug designing. Rohu DMRT1 was observed to interact with other proteins such as FOXL2, CYP19a1a, AMH and SOX9a. Differential expression study revealed higher expression in testis tissue implying its role in male sex differentiation and testicular development. The information generated in the present work could facilitate further research to resolve the issues related to gonadal maturation and reproduction of commercially important aquaculture species.

摘要

鱼类形态、生理和行为的两性二态性在本质上是多样而复杂的。Doublesex 和mab-3 相关转录因子( Dmrt ) 是一个大型蛋白质家族，其功能是脊椎动物的有性发育和分化。在这里，我们报告了Labeo rohita (rohu) Dmrt1的全长 cDNA 序列，长度为 907 bp，具有 798 bp 的开放阅读框，编码 265 个氨基酸。发现rohu DMRT1蛋白的分子量为28.74 KDa，等电点为7.53。DMRT1 蛋白含有 23 个带正电荷和 24 个带负电荷的氨基酸，GRAVY 评分为 -0.618。在 DMRT1 蛋白中发现了一个特征性的 DM 结构域，它是一个新的 DNA 结合结构域。系统发育分析显示与Cyprinus carpio与其他脊椎动物的DMRT1相比。分子对接研究确定了药物设计的目标活性位点。观察到 Rohu DMRT1 与其他蛋白质相互作用，如 FOXL2、CYP19a1a、AMH 和 SOX9a。差异表达研究揭示睾丸组织中更高的表达，暗示其在男性性别分化和睾丸发育中的作用。当前工作中产生的信息可以促进进一步研究，以解决与具有重要商业价值的水产养殖物种的性腺成熟和繁殖相关的问题。