The maternal immune system tolerates semi-allogeneic placental tissues during pregnancy. Fas ligand (FASLG) and tumor necrosis factor superfamily 10 (TNFSF10) are known to be components of maternal immune tolerance in humans and mice. However, the role of FASLG and TNFSF10 in the tolerance process has not been studied in pigs, which form a true epitheliochorial type placenta. Thus, the present study examined the expression and function of FASLG and TNFSF10 and their receptors at the maternal-conceptus interface in pigs. The endometrium and conceptus tissues expressed FASLG and TNFSF10 and their receptor mRNAs during pregnancy in a stage-specific manner. During pregnancy, FASLG and TNFSF10 proteins were localized predominantly to endometrial luminal epithelial cells with strong signals on Day 30 to term and on Day 15, respectively, and receptors for TNFSF10 were localized to some stromal cells. Interferon-γ (IFNG) increased the expression of TNFSF10 and FAS in endometrial tissues. Co-culture of porcine endometrial epithelial cells over-expressing TNFSF10 with peripheral blood mononuclear cells yielded increased apoptotic cell death of lymphocytes and myeloid cells. In addition, many apoptotic T cells were found in the endometrium on Day 15 of pregnancy. The present study demonstrated that FASLG and TNFSF10 were expressed at the maternal-conceptus interface and conceptus-derived IFNG increased endometrial epithelial TNFSF10, which, in turn, induced apoptotic cell death of immune cells. These results suggest that endometrial epithelial FASLG and TNFSF10 may be critical for the formation of micro-environmental immune privilege at the maternal-conceptus interface for the establishment and maintenance of pregnancy in pigs.

中文翻译：

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肿瘤坏死因子超家族的子宫上皮表达：一种真正的上皮绒毛胎盘物种妊娠期间免疫特权的策略

母体免疫系统在怀孕期间耐受半同种异体胎盘组织。Fas 配体 (FASLG) 和肿瘤坏死因子超家族 10 (TNFSF10) 是人类和小鼠母体免疫耐受的组成部分。然而，尚未在猪中研究 FASLG 和 TNFSF10 在耐受过程中的作用，猪形成真正的上皮细胞型胎盘。因此，本研究检查了 FASLG 和 TNFSF10 及其受体在猪母体 - 概念界面上的表达和功能。子宫内膜和孕体组织在妊娠期间以特定阶段的方式表达 FASLG 和 TNFSF10 及其受体 mRNA。在怀孕期间，FASLG 和 TNFSF10 蛋白主要定位于子宫内膜腔上皮细胞，分别在第 30 天至足月和第 15 天具有强信号，和 TNFSF10 受体定位于一些基质细胞。干扰素-γ（IFNG）增加了子宫内膜组织中 TNFSF10 和 FAS 的表达。过表达 TNFSF10 的猪子宫内膜上皮细胞与外周血单核细胞共培养导致淋巴细胞和骨髓细胞的凋亡细胞死亡增加。此外，在怀孕第 15 天的子宫内膜中发现了许多凋亡 T 细胞。本研究表明 FASLG 和 TNFSF10 在母体 - 概念界面表达，并且来自概念的 IFNG 增加子宫内膜上皮 TNFSF10，进而诱导免疫细胞的凋亡细胞死亡。