As part of the species recovery plan for the critically endangered pygmy hog (Porcula salvania), a conservation-breeding program was initiated, to bolster its wild population. For successful conservation-breeding, it is essential to maintain 90% of the founder genetic diversity over time. Therefore, in the present study, we assessed the genetic diversity of a captive population of pygmy hog across generations using a set of ten, cross-specific microsatellite markers. Our results indicated a genetically heterozygous captive population (H_E = 0.603), with stable expected heterozygosities across generations. However, the most recent generation showed a significant decrease in individual heterozygosities, implying possible genetic inbreeding. The current findings warrant a need for genetic evaluation to inform future conservation-breeding decisions. In addition, we also designed and tested primers for PCR-based species and sex-identification in the pygmy hog. The markers standardised in the present study would also help in evaluating the survival and ecology of the reintroduced populations.

作为极度濒危的侏儒猪（Porcula salvania）物种恢复计划的一部分，发起了一项保护繁殖计划，以加强其野生种群。为了成功进行保护育种，至关重要的是要随着时间的推移保持创始人的90％的遗传多样性。因此，在本研究中，我们使用一组十个交叉特异性微卫星标记评估了圈养侏儒猪世代间的遗传多样性。我们的结果表明，遗传杂合的圈养种群（H _E = 0.603），且各代人的预期杂合度均稳定。但是，最新一代显示出个体杂合度的显着降低，这意味着可能存在遗传近交。目前的发现需要进行遗传评估，以为将来的保护育种决策提供依据。此外，我们还为侏儒猪中基于PCR的物种和性别鉴定设计并测试了引物。在本研究中标准化的标记还将有助于评估重新引入种群的生存和生态。