Abstract

Doxorubicin (DOX) has been linked with impairment in cardiovascular function and redox balance. In the present study, the effect of Phyllanthus amarus (PA) and Momordica charantia (MC) leaves on some biomolecules [Angiotensin-I converting enzyme (ACE), arginase, acetylcholinesterase (AChE), adenosine deaminase (ADA), lactate dehydrogenase (LDH)] and antioxidant [catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA)] linked with cardiac function in DOX-stressed rats was evaluated. Animals were grouped and pretreated with PA and MC leaf extracts at different doses (200 and 400 mg/kg/bwt orally), while DOX (15 mg/kg/bwt) was administered intraperitoneally on the last day of the experiment. Result revealed an increase of ACE, arginase, AChE, ADA, LDH activities and MDA level as well as a significant reduction in CAT and SOD activities, and GSH level in the rats treated with DOX compared to the control. However, these were significantly mitigated in the rats pretreated with PA and MC dose dependently. Chemical characterization of the leaf extracts via high performance liquid chromatography revealed the presence of some phenolic compounds which included kaempferol, catechin, epicatechin, ellagic acid, gallic acid quercetin, isoquercitrin and rutin. These findings revealed a significant improvement in redox imbalance and other biomolecules associated with cardiac function, which was altered by DOX. This improvement could be linked to the presence of cardioprotective agents present in PA and MC, thereby making these plants therapeutic agents for the treatment of cardiovascular complications associated with drugs such as DOX.

摘要

多柔比星 (DOX) 与心血管功能和氧化还原平衡受损有关。在本研究中，Phyllanthus amarus (PA) 和苦瓜的作用(MC) 叶在一些生物分子 [血管紧张素-I 转换酶 (ACE)、精氨酸酶、乙酰胆碱酯酶 (AChE)、腺苷脱氨酶 (ADA)、乳酸脱氢酶 (LDH)] 和抗氧化剂 [过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、评估了与 DOX 应激大鼠心脏功能相关的谷胱甘肽 (GSH) 和丙二醛 (MDA)]。将动物分组并用不同剂量的 PA 和 MC 叶提取物（口服 200 和 400 mg/kg/bwt）进行预处理，而在实验的最后一天腹膜内施用 DOX（15 mg/kg/bwt）。结果显示，与对照组相比，用 DOX 治疗的大鼠的 ACE、精氨酸酶、AChE、ADA、LDH 活性和 MDA 水平升高，CAT 和 SOD 活性以及 GSH 水平显着降低。然而，这些在用 PA 和 MC 剂量依赖性预处理的大鼠中显着减轻。通过高效液相色谱对叶提取物的化学表征揭示了一些酚类化合物的存在，包括山奈酚、儿茶素、表儿茶素、鞣花酸、没食子酸槲皮素、异槲皮苷和芦丁。这些发现揭示了氧化还原失衡和与心脏功能相关的其他生物分子的显着改善，而 DOX 改变了心脏功能。这种改善可能与 PA 和 MC 中存在心脏保护剂有关，从而使这些植物成为治疗与 DOX 等药物相关的心血管并发症的治疗剂。通过高效液相色谱对叶提取物的化学表征揭示了一些酚类化合物的存在，包括山奈酚、儿茶素、表儿茶素、鞣花酸、没食子酸槲皮素、异槲皮苷和芦丁。这些发现揭示了氧化还原失衡和与心脏功能相关的其他生物分子的显着改善，而 DOX 改变了心脏功能。这种改善可能与 PA 和 MC 中存在心脏保护剂有关，从而使这些植物成为治疗与 DOX 等药物相关的心血管并发症的治疗剂。通过高效液相色谱对叶提取物的化学表征揭示了一些酚类化合物的存在，包括山奈酚、儿茶素、表儿茶素、鞣花酸、没食子酸槲皮素、异槲皮苷和芦丁。这些发现揭示了氧化还原失衡和与心脏功能相关的其他生物分子的显着改善，而 DOX 改变了心脏功能。这种改善可能与 PA 和 MC 中存在心脏保护剂有关，从而使这些植物成为治疗与 DOX 等药物相关的心血管并发症的治疗剂。这些发现揭示了氧化还原失衡和与心脏功能相关的其他生物分子的显着改善，而 DOX 改变了心脏功能。这种改善可能与 PA 和 MC 中存在心脏保护剂有关，从而使这些植物成为治疗与 DOX 等药物相关的心血管并发症的治疗剂。这些发现揭示了氧化还原失衡和与心脏功能相关的其他生物分子的显着改善，而 DOX 改变了心脏功能。这种改善可能与 PA 和 MC 中存在心脏保护剂有关，从而使这些植物成为治疗与 DOX 等药物相关的心血管并发症的治疗剂。