DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication,Journal of the Science of Food and Agriculture

当前位置： X-MOL 学术 › J. Sci. Food Agric. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication
Journal of the Science of Food and Agriculture ( IF 3.3 ) Pub Date : 2020-02-14 , DOI: 10.1002/jsfa.10241
Yanfei Ding ₁ , Guangze Jiang ₁ , Lihong Huang ₁ , Chengtong Chen ₁ , Junwei Sun ₁ , Cheng Zhu ₁

Affiliation

BACKGROUND Walnut (Juglans regia L.) is one of the most widely cultivated nuts. Walnut milk beverage is very popular in China due to its nutritional value. However, adulterated walnut milk ingredients have been detected in the Chinese market. Peanut and soybean are sold at much lower prices than walnut and are reported as common adulterated species in the industrial chain of walnut milk production. Therefore, the purpose of this study is to develop an accurate and efficient method for detecting the authenticity of raw materials of walnut milk beverage. RESULTS DNA barcoding and high-resolution melting (HRM) analysis were used to identify common adulterated raw ingredients such as peanut and soybean in commercial walnut milk beverage samples. The chloroplast psbA-trnH gene was used for sequencing, and HRM analysis was performed. We also prepared in laboratory experimental mixtures with different quantities of walnut, peanut and soybean. HRM analysis of the experimental mixtures clearly distinguished all of them. Results revealed that most of the walnut milk beverage samples fell in the same cluster of walnut species. Several samples fell in the cluster of peanut, confirming that they were adulterated walnut milk products. CONCLUSION The results revealed that HRM analysis based on the psbA-trnH barcode sequence can be used to identify raw ingredients in walnut milk beverages. This article is protected by copyright. All rights reserved.

更新日期：2020-02-14

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11