Airborne pollen is an important and potent source of allergens. Allergenic protein components located in the cytoplasm of pollen, so-called pollen cytoplasmic granules, are released upon contact with moisture, thereby inducing allergic symptoms. However, few studies have compared factors affecting pollen rupture and protein release from allergenic pollen. This study investigated the dynamics of pollen rupture and protein release upon hydration using common allergenic grass and weed species in subtropical regions. The three factors examined were incubation time, incubating solution, and pollen age after shedding. We found that pollen rupture and protein release occurred rapidly upon contact with solutions. The highest amount of protein was released within 15 min after incubation. As expected, pollen rupture increased as incubation time increased. Fresh pollen had higher rupture percentage and released a higher amount of protein than aged pollen. Rainwater caused more rupture and triggered more protein release than other tested solutions. The dynamics of rupture and the amount of released protein were drastically different among species. In conclusion, incubation time, incubating solution, and age of pollen significantly affected the amount of pollen rupture and protein release from allergenic pollen. This information provided better understanding of factors involving the release of allergenic protein from pollen in relation to its internal and external conditions, which could affect its allergenicity.

中文翻译：

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亚热带地区常见的过敏性草和杂草水合后花粉快速破裂和花粉细胞质颗粒的释放

空气中的花粉是过敏原的重要而有效的来源。位于花粉细胞质中的致敏蛋白成分，即所谓的花粉细胞质颗粒，在接触水分后会释放，从而诱发过敏症状。然而，很少有研究比较影响花粉破裂和过敏性花粉蛋白质释放的因素。本研究使用亚热带地区常见的致敏草和杂草物种研究了水合作用后花粉破裂和蛋白质释放的动态。检查的三个因素是孵化时间、孵化溶液和脱落后的花粉年龄。我们发现花粉破裂和蛋白质释放在与溶液接触后迅速发生。孵育后 15 分钟内释放出最高量的蛋白质。正如预期的那样，花粉破裂随着孵化时间的增加而增加。与陈年花粉相比，新鲜花粉具有更高的破裂率并释放更多的蛋白质。与其他测试溶液相比，雨水会导致更多的破裂并引发更多的蛋白质释放。破裂的动力学和释放的蛋白质的数量在物种之间有很大的不同。总之，培养时间、培养液和花粉年龄显着影响花粉破裂的数量和致敏花粉的蛋白质释放量。该信息提供了对涉及从花粉中释放过敏原蛋白与其内部和外部条件相关的因素的更好理解，这些因素可能影响其过敏性。破裂的动力学和释放的蛋白质的数量在物种之间有很大的不同。总之，培养时间、培养液和花粉年龄显着影响花粉破裂的数量和致敏花粉的蛋白质释放量。该信息提供了对涉及从花粉中释放过敏原蛋白与其内部和外部条件相关的因素的更好理解，这些因素可能影响其过敏性。破裂的动力学和释放的蛋白质的数量在物种之间有很大的不同。总之，培养时间、培养液和花粉年龄显着影响花粉破裂的数量和致敏花粉的蛋白质释放量。该信息提供了对涉及从花粉中释放过敏原蛋白与其内部和外部条件相关的因素的更好理解，这些因素可能影响其过敏性。