ABSTRACT

A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members.

 抽象的

越来越多的tet(X)型替加环素耐药决定簇 [ tet(X1)至tet(X5) ] 构成了一个不断扩大的四环素失活酶家族，对全球公共卫生构成潜在风险。在这里，我们报告了一种有效的多重 PCR 方法的开发，用于检测tet(X)变体家族。该方法成功应用于现场和临床细菌样本中tet(X)基因的筛选和验证。此外，我们发现之前提出的tet(X1)是由于不适当的注释而过早截断的版本，并修复了此错误。总的来说，它可能是第一个用于检测不同 Tet(X) 成员的遗传工具。