One of the major factors that influences the economic feasibility of biogas production is the availability of digestible feedstocks. There is little research on the influence of the chemical composition of biomass on biogas synthesis, especially with regard to the content of lignocellulosic materials. Therefore, the aim of this study was to estimate how differences in the content of cellulose and lignin in lignocellulosic biomass influence the concentrations of individual volatile fatty acids (VFAs) and biogas production. Additionally, the structure of the methanogenic community was examined. The removal of fibrous and non-fibrous materials, the concentrations of individual VFAs, methane production and methanogen community structure were examined during digestion of Zea mays L. and Miscanthus sacchariflorus silages. Organics were removed with higher efficiency during the digestion of Z. mays silage than during digestion of M. sacchariflorus. This was due to the higher non-fibrous carbohydrates content in Z. mays than in M. sacchariflorus. In both digesters, propionate predominated throughout experiment. The methanogenic community in the digester fed with Z. mays was more diverse than that in the digester with M. sacchariflorus. Analysis of 16S rRNA sequences showed that six acetoclastic and four hydrogenotrophic methanogens were present in the digester fed with Z. mays L., while five acetoclastic and three hydrogenotrophic methanogens were in the digester fed with M. sacchariflorus. The abundance of Methanosarcina correlated significantly with the concentration of all analyzed VFAs.

中文翻译：

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木质纤维素生物质的各个组成成分对甲烷生产和产甲烷菌群落结构的影响

影响沼气生产经济可行性的主要因素之一是可消化原料的可用性。关于生物质的化学组成对沼气合成的影响的研究很少，特别是在木质纤维素材料的含量方面。因此，本研究的目的是评估木质纤维素生物质中纤维素和木质素含量的差异如何影响单个挥发性脂肪酸（VFA）的浓度和沼气的产生。另外，检查了产甲烷菌群落的结构。在玉米和玉米消化过程中检查了纤维和非纤维材料的去除，各个VFA的浓度，甲烷的产生和产甲烷菌的群落结构。芒草青贮饲料。在消化玉米青贮青贮过程中，去除有机物的效率比消化糖藻时高。这是由于在较高的非纤维碳水化合物含量玉蜀黍比M. sacchariflorus。在两个消化池中，整个实验过程中丙酸占主导地位。饲喂玉米（Z. mays）的消化器中产甲烷的群落比糖酵母（M. sacchariflorus）的消化器中的产甲烷菌更多样化。对16S rRNA序列的分析表明，在饲喂玉米digest虫的消化池中存在6种破骨细胞和4种氢营养型产甲烷菌。L.，而5和乙酸分解和三个氢营养甲烷分别与供给蒸煮M. sacchariflorus。甲烷八叠球菌的丰度与所有分析的VFA的浓度显着相关。