Objective

Solid lipid nanoparticles (SLN) mainly consist of lipids and surfactants. However, the toxicity of surfactant cannot be ignored, as the surfactant may cause unexpected side effect. This study established model SLN with different surfactants, Tween 80 (T80) and poloxamer 188 (P188). It was aimed to establish ultraviolet-visible (UV-Vis) spectroscopy methods for quantifying the contents of T80 and P188 in the model SLN.

Methods

The model SLN were produced by emulsification-precipitation method, and their particle size distribution was analyzed. The calibration curves for the contents of T80 and P188 were established. Surfactants in the model SLN were extracted by ultrasonication, and UV-Vis spectroscopy methods were utilized for quantification of surfactants in SLN.

Results

The deviation of precision and accuracy in P188 and T80 assay were acceptable, which indicated the method could be employed in sample quantification. Ten minutes was considered as the optimal ultrasonication time. And the contents of P188 and T80 were successfully measured by the established UV-Vis methods.

Conclusion

The UV-Vis spectroscopy methods were developed for the quantification of T80 and P188 in the model SLN. It is expected that the methods could be extended to more SLN systems and applied for the quantification of other surfactants.

中文翻译：

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固体脂质纳米颗粒中表面活性剂的光谱定量

目的

固体脂质纳米颗粒（SLN）主要由脂质和表面活性剂组成。但是，表面活性剂的毒性不可忽视，因为表面活性剂可能会引起意想不到的副作用。这项研究建立了具有不同表面活性剂Tween 80（T80）和泊洛沙姆188（P188）的SLN模型。目的是建立紫外可见（UV-Vis）光谱法，以定量SLN模型中T80和P188的含量。

方法

通过乳化沉淀法制备了SLN模型，并对其粒度分布进行了分析。建立了T80和P188含量的校准曲线。通过超声提取SLN模型中的表面活性剂，并使用UV-Vis光谱法定量SLN中的表面活性剂。

结果

在P188和T80分析中，精密度和准确性的偏差是可以接受的，这表明该方法可用于样品定量。十分钟被认为是最佳超声处理时间。通过建立的UV-Vis方法成功地测定了P188和T80的含量。

结论

开发了UV-Vis光谱法对SLN模型中的T80和P188进行定量。预期该方法可以扩展到更多的SLN系统，并用于其他表面活性剂的定量。