Kenaf is one of the most important natural fiber crops. The lack of molecular markers has resulted in a large gap in the improvement of kenaf. Previously, de novo 90,175 unigenes in kenaf were assembled. In this study, 7453 small InDels were called from these uingenes. The indel size <5 bp, 5-10 bp and > 10 bp have frequencies of 71.50%, 23.05%, and 5.45%. 337 InDels locating in genes encoding transcription factors were used to survey polymorphisms with 24 kenaf accessions. Of 337 InDels, 305 (90.5%) successfully amplified at least one fragment and detected polymorphism among the 24 diverse genotypes. Furthermore, one InDel marker, HcID266, was co-segregated with leaf shape, which was inherited as a single Mendelian locus, in the F₂ population with 390 individuals. To our knowledge, this is the first large-scale InDels development for kenaf. These InDel markers will be helpful for genetic researches in kenaf.

中文翻译：

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InDel标记物的开发用于鉴定控制红麻（Hibiscus cannabinus）叶片形状的单个孟德尔位点。

洋麻是最重要的天然纤维作物之一。分子标记物的缺乏导致了洋麻的改良存在很大差距。以前，在洋麻中重新组装了90,175个单基因。在这项研究中，从这些烯中调用了7453个小的InDel。<5 bp，5-10 bp和> 10 bp的插入缺失大小的频率分别为71.50％，23.05％和5.45％。位于编码转录因子基因中的337个InDels被用于调查24个红麻种质的多态性。在337个InDels中，有305个（90.5％）成功扩增出至少一个片段，并在24种不同基因型中检测出多态性。此外，在F _2中，一种InDel标记物HcID266与叶片形状共分离，而叶片形状是作为单个孟德尔基因座遗传的。人口390人。据我们所知，这是洋麻的第一个大规模InDels开发。这些InDel标记将有助于洋麻的遗传研究。