Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions.,Insect Molecular Biology

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Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-01-13 , DOI: 10.1111/imb.12631
P Thorpe ₁ , C M Escudero-Martinez _{1,

2} , S Eves-van den Akker ₃ , J I B Bos _{1,

2}

Affiliation

Aphids feature complex life cycles, which in the case of many agriculturally important species involve primary and secondary host plant species. Whilst host alternation between primary and secondary host can occur in the field depending on host availability and the environment, aphid populations maintained as laboratory stocks generally are kept under conditions that allow asexual reproduction by parthenogenesis on secondary hosts. We used Myzus cerasi (black cherry aphid) to assess aphid transcriptional differences between populations collected from primary hosts in the field and those adapted to secondary hosts under controlled environment conditions. Transfer of M. cerasi collected from local cherry trees to reported secondary host species resulted in low survival rates. Moreover, aphids were unable to survive on the secondary host land cress, unless first adapted to another secondary host, cleavers. Transcriptome analyses of the different aphid populations (field collected and adapted) revealed extensive transcriptional plasticity to a change in environment, with predominantly genes involved in redox reactions differentially regulated. Most of the differentially expressed genes were duplicated and we found evidence for differential exon usage. Our data suggest that aphid adaptation to different environments may pose a major hurdle and leads to extensive gene expression changes.

中文翻译：

不同宿主和环境条件下蚜虫Myzus cerasi的转录变化。

蚜虫具有复杂的生命周期，在许多农业重要物种的情况下，涉及初级和次级宿主植物物种。虽然取决于宿主可用性和环境，主要宿主和次要宿主之间的宿主交替可以在田间发生，但作为实验室种群维持的蚜虫种群通常保持在允许通过在次要宿主上通过孤雌生殖进行无性繁殖的条件下。我们使用Myzus cerasi（黑樱桃蚜虫）来评估从田间主要宿主收集的种群与在受控环境条件下适应次要宿主的种群之间的蚜虫转录差异。M的转移。切拉西从当地樱桃树收集到报告的次要宿主物种导致低存活率。此外，蚜虫无法在次要宿主陆地水芹上生存，除非首先适应另一个次要宿主，即切菜刀。对不同蚜虫种群（田间收集和适应）的转录组分析揭示了对环境变化的广泛转录可塑性，主要是参与氧化还原反应的基因受到差异调节。大多数差异表达的基因被复制，我们发现了差异外显子使用的证据。我们的数据表明，蚜虫对不同环境的适应可能构成主要障碍并导致广泛的基因表达变化。

更新日期：2020-01-13

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