This study compared the microtensile bond strength (BS) and the micromorphology of resin-dentin interface (MI) of a conventional adhesive to two-steps etch-and-rinse bonding agents after 1 year of water storage. Twenty-eight human third molars were used (n = 7). Teeth were divided into four groups (GCB: Gluma Comfort Bond; OPB: OptiBond FL; OCB: One Coat Bond SL; PUB: Peak Universal Bond). Specimens were tested in tension after 24 h or 1 year of water storage. Dentin BS strength data were analyzed by split-plot two-way ANOVA followed by Tukey–Kramer tests (α = 0.05). Water-storage for 1 year significantly decreased BS for OCB; however, no significant difference was noted between OCB and GCB and PUB adhesives after 1 year of water-storage. OPB showed the highest BS values at both storage times. All adhesives formed a hybrid layer with resin tags, and both interfacial structures were maintained after 1 year. However, degradation signals within interfacial structures were observed only for the adhesive with a bond strength reduction. BS test showed that only one adhesive declined after 1 year of water storage. The degradation of some structures of the interface collaborated this finding. The classic three-step etch-and-rinse (OPB) presented higher values of bond strength than GCB and OCB after 1 year, but it was not statistically different from PUB.

中文翻译：

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贮水1年后蚀刻和冲洗型牙本质粘合剂的树脂-牙本质界面的粘合强度和微观形态

这项研究比较了传统粘合剂与蓄水1年后的两步蚀刻和漂洗粘合剂的微拉伸粘合强度（BS）和树脂-牙本质界面（MI）的微观形态。使用了28个人类第三颗臼齿（n = 7）。牙齿分为四组（GCB：Gluma舒缓胶； OPB：OptiBond FL； OCB：一层涂层胶SL； PUB：Peak Universal Bond）。储水24小时或1年后，对样品进行张力测试。牙本质BS强度数据的分析采用分裂图双向方差分析，然后采用Tukey–Kramer检验（α= 0.05）。蓄水1年，OCB的BS显着降低；但是，储水1年后，OCB和GCB和PUB粘合剂之间没有显着差异。OPB在两个存储时间均显示出最高的BS值。所有胶粘剂形成了带有树脂标签的混合层，1年后，两个界面结构都得以保留。然而，仅对于粘合强度降低的粘合剂，观察到界面结构内的降解信号。BS测试表明，储水1年后只有一种胶粘剂下降。界面某些结构的退化与这一发现相辅相成。1年后，经典的三步蚀刻和冲洗（OPB）的结合强度值高于GCB和OCB，但与PUB在统计学上没有差异。