The aim of this study was to improve the rooting efficiency of Eucalyptus urophylla clones by in vitro reinvigoration/rejuvenation in two clones (02 and 04) from the breeding program of the V&M Florestal company. An in vitro culture began with 200 meristems of each clone, which were excised, disinfected, and inoculated in culture medium. When shoots from these first meristems inoculated reached a height of 3 cm, 100 new meristematic regions of 0.5 cm were isolated and inoculated in culture medium. The other shoots from were inoculated in a rooting medium, where they remained for 30 days. After this period, the plants were acclimatized and used as stock plants for shoot production in a commercial nursery. This process was repeated until the shoots attained an ex vitro rooting rate of more than 80%. After reinvigoration/rejuvenation of clones 02 and 04, the relationship between rooting and the presence of starch and phenolic compounds at the base of the minicuttings was histochemically analyzed. For clone 02, three in vitro subcultures were needed to increase the rooting rate, and for clone 04, only one in vitro subculture was required. In vitro reinvigoration/rejuvenation is a determining factor for greater rooting efficiency of minicuttings of 02 and 04 clones. Production of sclerenchyma fibers around the root vascular cylinder and starch and phenolic compound production are directly related to rooting efficiency.

中文翻译：

---

体外系列继代培养以改善尾叶桉的生根

这项研究的目的是提高尾叶桉的生根效率通过在V＆M Florestal公司的育种程序中将两个克隆（02和04）进行体外再生/再生来克隆这些克隆。体外培养开始于每个克隆的200个分生组织，将其切出，消毒并接种在培养基中。当这些最初分生组织的枝条接种到3 cm的高度时，分离出100个0.5 cm的新分生组织区域，并将其接种在培养基中。将来自的其他芽接种在生根培养基中，并在其中保留30天。在此期间之后，使植物适应环境并用作商业苗圃中用于生产芽的储备植物。重复该过程，直到枝条的离体生根率超过80％。重新激活/恢复克隆02和04后，组织化学分析了生根与小切口根部淀粉和酚类化合物的存在之间的关系。对于克隆02，需要进行三个体外传代培养以提高生根率，对于克隆04，仅需要进行一个体外传代培养。体外再生/恢复活力是决定02和04克隆小插倍生根效率的决定因素。根血管周围的巩膜纤维的产生以及淀粉和酚类化合物的产生与生根效率直接相关。体外再生/恢复活力是决定02和04克隆小插倍生根效率的决定因素。根血管周围的巩膜纤维的产生以及淀粉和酚类化合物的产生与生根效率直接相关。体外再生/恢复活力是决定02和04克隆小插倍生根效率的决定因素。根血管周围的巩膜纤维的产生以及淀粉和酚类化合物的产生与生根效率直接相关。