The optimization of factors affecting methane production from the co-digestion of algal biomass with crude glycerol was investigated using response surface methodology with a central composite design. The investigated parameters included algal biomass, crude glycerol and inoculum concentration. The inoculum and algal biomass concentration had a significant individual effect on the methane production (p ≤ 0.05). The interactive effect on methane production was found to be between algal biomass and crude glycerol (p ≤ 0.05). The optimal conditions were 20.02 g-VS/L of crude glycerol, 9.76 g-VS/L of inoculum concentration and 5.50 g-VS/L of algal biomass. Under optimum conditions, the maximum methane production of 58.88 mL-CH₄/L was obtained. The difference between the observed methane production (58.88 mL-CH₄/L) and the predicted methane (68.94 mL-CH₄/L) was 14.59%. Under the optimum conditions, the energy production of 92.47 J/g-VS_added was obtained. The polymerase chain reaction-denaturing gradient gel electrophoresis analysis indicated that the methane producers present in the fermentation broth were Methanosarcina sp., Methanoregula sp., Methanospirillum sp. and Methanoculleus sp.

中文翻译：

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厌氧混合培养法将藻类生物质与甘油粗消化而产生的甲烷

使用响应表面方法和中心复合设计，研究了藻类生物质与粗甘油共消化过程中影响甲烷生产的因素的优化。研究的参数包括藻类生物量，粗甘油和接种物浓度。接种物和藻类生物质浓度对甲烷产生（一显著个体效应p  ≤0.05）。对甲烷产生互动效应被发现是藻类生物质和粗甘油（间p  ≤0.05）。最佳条件是粗甘油为20.02 g-VS / L，接种物浓度为9.76 g-VS / L，藻类生物质为5.50 g-VS / L。在最佳条件下，最大甲烷生成量为58.88 mL-CH ₄获得/ L。观察到的甲烷产量（58.88 mL-CH ₄ / L）与预测的甲烷（68.94 mL-CH ₄ / L）之间的差异为14.59％。在最佳条件下，能量生产92.47焦耳/克-VS的_加入得到。聚合酶链反应-变性梯度凝胶电泳分析表明，发酵液中存在的甲烷产生者是甲烷菌属，甲烷菌属，甲烷螺旋菌属。和Methanoculleus sp。