The blister blight disease caused by the fungus, Exobasidium vexans has serious implications on the quality of tea production. The disease however, has been poorly studied and hence there is very limited information on the pathogen and as such the pathogenesis of blister blight infection. One of the major roadblocks in understanding E. vexans is the obligate and biotrophic nature of the fungus which limits the establishment and maintenance of in vitro cultures. To address this issue, a Central Composite Design based Response Surface Methodology (RSM) was adopted to study the modification of three fungal culture media viz. czapek dox, potato dextrose, and v8 juice, and the effect of altered media composition on growth conditions and media compositions were assessed. The response parameter for the RSM experiments was the mycelial biomass produced under different culture conditions. The uni and bi-parametric interactions among the experimental variables provided the basis for the statistically optimized conditions for maximal fungal growth. The study thus presents the recommended modifications of existing media that can lead to the successful establishment and maintenance of E. vexans in vitro cultures.

中文翻译：

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通过响应面法优化生物营养性真菌Exobasidium v​​exans的体外培养条件

由真菌Exobasidium v​​exans引起的水泡枯萎病严重影响了茶叶的生产质量。然而，对该疾病的研究还很差，因此关于病原体以及水疱枯萎病感染的发病机理的信息非常有限。了解埃克森州大肠杆菌的主要障碍之一是真菌的专性和生物营养性质，限制了体外培养物的建立和维持。为了解决这个问题，采用了基于中央复合设计的响应面方法（RSM）来研究三种真菌培养基的改良。评估了czapek dox，马铃薯葡萄糖和v8汁液，以及改变培养基成分对生长条件和培养基成分的影响。RSM实验的响应参数是在不同培养条件下产生的菌丝体生物量。实验变量之间的单参数和双参数相互作用为最大真菌生长的统计优化条件提供了基础。因此，该研究提出了对现有媒体的建议修改，可以成功建立和维护E. vexans体外培养。