Single-cell isolation and cloning are essential steps in many applications, ranging from the production of biotherapeutics to stem cell therapy. Having confidence in monoclonality in such applications is essential from both research and commercial perspectives, for example, to ensure that data are of high quality and regulatory requirements are met. Consequently, several approaches have been developed to improve confidence in monoclonality. However, ensuring monoclonality using standard well plate formats remains challenging, primarily due to edge effects; the solid wall around a well can prevent a clear view of how many cells might be in a well. We describe a method that eliminates such edge effects: solid confining walls are replaced by transparent fluid ones, and standard low-cost optics can confirm monoclonality.

中文翻译：

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使用流体壁进行单细胞克隆可确保单克隆性。

从生物疗法的生产到干细胞疗法，单细胞分离和克隆是许多应用中必不可少的步骤。从研究和商业角度看，对此类应用的单克隆性都有信心，例如，以确保数据具有高质量并满足法规要求。因此，已经开发了几种方法来提高对单克隆性的信心。然而，主要由于边缘效应，使用标准孔板形式确保单克隆性仍然具有挑战性。孔周围的实心壁可能无法清晰显示孔中可能有多少个细胞。我们描述了一种消除这种边缘效应的方法：用透明的流体壁代替固体约束壁，而标准的低成本光学器件可以确认单克隆性。