Lumbar puncture (LP) is a common way of collecting cerebrospinal fluid (CSF) both in the clinic and in research. In this extension of a study on the relationship between sleep deprivation and CSF biomarkers for Alzheimer’s disease, we investigated CSF biomarker dynamics in relation to rebound sleep after sleep deprivation. Two LPs were performed within 3 days in 13 healthy volunteers. We noticed an unexpected sharp rise in biomarker concentrations in the second sample and therefore repeated the experiment, but without sleep intervention, in four additional individuals. The findings were similar in these subjects, suggesting an inherent methodological problem with repeated LPs. The result corroborates findings in studies with repeated CSF collection via indwelling lumbar catheters, and needs to be addressed in, for instance, pharmacodynamic studies employing these techniques.

中文翻译：

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3天内重复腰椎穿刺可能会影响脑脊液生物标志物水平

腰椎穿刺 (LP) 是临床和研究中收集脑脊液 (CSF) 的常用方法。在这项关于睡眠剥夺与阿尔茨海默病脑脊液生物标志物之间关系的研究的扩展中，我们研究了与睡眠剥夺后睡眠反弹相关的脑脊液生物标志物动态。13 名健康志愿者在 3 天内进行了两次 LP。我们注意到第二个样本中生物标志物浓度出乎意料地急剧上升，因此在另外四个人中重复了实验，但没有睡眠干预。在这些受试者中的发现是相似的，表明重复 LP 存在固有的方法学问题。结果证实了通过留置腰椎导管重复收集脑脊液的研究结果，需要在例如，