The aim of this work is to provide a comprehensive and unbiased understanding at the molecular correlates of peripheral nerve injury. In this study, we screened the differentially expressed genes (DEGs) in the DRG from rats using RNA-seq technique. Moreover, the bioinformatics methods were used to figure out the signaling pathways and expression regulation pattern of the DEGs enriched in. In addition, quantitative real-time RT-PCR was carried out to further confirm the expression of DEGs. 414 genes were upregulated, while 184 genes were downregulated in the DRG of rats 7 days after partial sciatic nerve ligation (pSNL) surgery. Moreover, GO and KEGG enrichment analysis suggested that most of the altered genes were involved in inflammatory responses and signaling transduction. In addition, our results state that they shared similar characters in the DRG among four types of neuropathic pain models. Eighteen genes have been altered (17 of them were upregulated) in the DRG of all four types of neuropathic pain models, in which Vgf, Atf3, Cd74, Gal, Jun, Npy, Serpina3n, and Hspb1 have been reported to be involved in neuropathic pain. Quantitative real-time RT-PCR results further confirmed the mRNA expression levels of Vgf, Atf3, Cd74, Gal, Jun, Npy, Serpina3n, and Hspb1 in the DRG of rats with pSNL surgery. The present study suggested that these eight genes may play important roles in neuropathic pain, revealing that these genes might serve as therapeutic targets for neuropathic pain. Moreover, anti-inflammatory therapy might be an effective approach for neuropathic pain treatment and prevention.

中文翻译：

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周围神经损伤后大鼠背根神经节疼痛的转录组学分析。

这项工作的目的是对周围神经损伤的分子相关性提供全面而公正的理解。在这项研究中，我们使用RNA序列技术从大鼠中筛选了DRG中的差异表达基因（DEG）。此外，利用生物信息学方法找出了富集的DEGs的信号传导途径和表达调控模式。此外，还进行了定量实时RT-PCR以进一步证实DEGs的表达。在部分坐骨神经结扎术（pSNL）手术后7天，DRG大鼠的414个基因被上调，而184个基因被下调。此外，GO和KEGG富集分析表明，大多数改变的基因与炎症反应和信号转导有关。此外，我们的结果表明，他们在四种类型的神经性疼痛模型中在DRG中具有相似的特征。在所有四种类型的神经性疼痛模型的DRG中，已经改变了18个基因（其中17个被上调），其中Vgf，Atf3，Cd74，Gal，Jun，Npy，Serpina3n和Hspb1已被报道与神经性疼痛有关。实时定量RT-PCR结果进一步证实了Vgf，Atf3，Cd74，Gal，Jun，Npy，Serpina3n和Hspb1的mRNA表达水平pSNL手术大鼠的DRG中的作用。本研究表明这八个基因可能在神经性疼痛中起重要作用，表明这些基因可能作为神经性疼痛的治疗靶标。此外，抗炎治疗可能是治疗和预防神经性疼痛的有效方法。