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Cloning of four HSPA multigene family members in Haemaphysalis flava ticks.
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-12-04 , DOI: 10.1111/mve.12423 L Liu 1 , X-M He 1 , L-L Feng 1 , D-Y Duan 1 , Y Zhan 1 , T-Y Cheng 1
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-12-04 , DOI: 10.1111/mve.12423 L Liu 1 , X-M He 1 , L-L Feng 1 , D-Y Duan 1 , Y Zhan 1 , T-Y Cheng 1
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The heat shock protein 70 (HSPA) family and their genes have been studied in ticks and are considered as possible antigen candidates for the development of anti-tick vaccines. However, knowledge about their members, structure and function in ticks is incomplete. Based on our transcriptomic data, the full length of four HSPA genes in Haemaphysalis flava (Acari: Ixodidae) was cloned via rapid amplification of cDNA ends. The open reading frame of HSPA2A, HSPA2B, HSPA5 and HSPA9 was 1920, 1911, 1983 and 2088 bp in length, respectively. Three family signatures and one localization motif were in the encoding proteins. HSPA2A and HSPA2B were predicted to be located at cytoplasm/nucleus, whereas HSPA5 and HSPA9 were at endoplasmic reticulum and mitochondria, respectively. In silico simulation demonstrated that those proteins had distinct numbers of α-helixes, extended strands and coils, and different antigenic epitopes. Expression of HSPA5 and HSPA9 in the salivary gland was significantly higher in partially-engorged female adult ticks than the fully-engorged (P < 0.01) as shown by a quantitative polymerase chain reaction. Our data indicated that H. flava ticks had at least four HSPA genes encoding proteins with different cellular locations, structures and expression profiles, suggesting their diverse roles in tick biology.
中文翻译：
血红素lava中四个HSPA多基因家族成员的克隆。
已经对shock进行了热休克蛋白70（HSPA）家族及其基因的研究，它们被认为是开发抗-疫苗的可能候选抗原。但是，关于their的成员，结构和功能的知识尚不完整。根据我们的转录组数据，通过快速扩增cDNA末端克隆了血红藻（Acari：Ixodidae）中四个HSPA基因的全长。HSPA2A，HSPA2B，HSPA5和HSPA9的开放阅读框的长度分别为1920、1911、1983和2088 bp。在编码蛋白中有三个家族特征和一个定位基序。HSPA2A和HSPA2B预计位于细胞质/细胞核，而HSPA5和HSPA9分别位于内质网和线粒体。在计算机模拟中显示，这些蛋白质具有不同数量的α-螺旋，延长的链和螺旋以及不同的抗原表位。定量聚合酶链反应显示，部分饱腹的成年雌性tick中唾液腺中HSPA5和HSPA9的表达显着高于完全饱腹的雌性（P <0.01）。我们的数据表明，H。flava壁虱具有至少四个编码具有不同细胞位置，结构和表达谱的蛋白质的HSPA基因，表明它们在壁虱生物学中的作用不同。
更新日期：2019-12-04
中文翻译：
血红素lava中四个HSPA多基因家族成员的克隆。
已经对shock进行了热休克蛋白70（HSPA）家族及其基因的研究，它们被认为是开发抗-疫苗的可能候选抗原。但是，关于their的成员，结构和功能的知识尚不完整。根据我们的转录组数据，通过快速扩增cDNA末端克隆了血红藻（Acari：Ixodidae）中四个HSPA基因的全长。HSPA2A，HSPA2B，HSPA5和HSPA9的开放阅读框的长度分别为1920、1911、1983和2088 bp。在编码蛋白中有三个家族特征和一个定位基序。HSPA2A和HSPA2B预计位于细胞质/细胞核，而HSPA5和HSPA9分别位于内质网和线粒体。在计算机模拟中显示，这些蛋白质具有不同数量的α-螺旋，延长的链和螺旋以及不同的抗原表位。定量聚合酶链反应显示，部分饱腹的成年雌性tick中唾液腺中HSPA5和HSPA9的表达显着高于完全饱腹的雌性（P <0.01）。我们的数据表明，H。flava壁虱具有至少四个编码具有不同细胞位置，结构和表达谱的蛋白质的HSPA基因，表明它们在壁虱生物学中的作用不同。
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