ABSTRACT

Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the development of periodontitis. The purpose of this investigation was to evaluate association of the MCP-1 gene polymorphism with chronic periodontitis in patients with end-stage renal disease (ESRD). One hundred fifty ESRD patients with chronic periodontitis (CP), 100 without CP and 190 healthy controls were included in this study. Genomic DNA from all participants was genotyped for the –2518 (A/G) polymorphism by a polymerase chain reaction – restriction fragment length polymorphism (PCR­-RFLP) assay. Significant differences were observed in the genotype and allele frequencies between patients with ESRD and CP and controls. The G allele frequency was significantly higher in patients than in control subjects, with odds ratio 1.77 (95 % CI 1.2–2.5), p = 0.0014. For the GG genotype the OR was 3.63 (95 % CI 1.5–8.76), p = 0.041. No significant differences in the polymorphism distribution were observed between ESRD patients without CP and control subjects. Comparison of the MCP-1 gene polymorphism distribution in ESRD patients with various primary diseases leading to ESRD did not show any significant differences. The mean MCP-1 serum levels were compared between subgroups. They were significantly higher in ESRD patients with CP (582 ± 112 pg/ml) than in patients without CP (309 ± 103 pg/ml) and controls (265 ± 85 pg/ml). Our results suggest that the MCP-1-2518 A/G polymorphism might be a novel risk factor for developing chronic periodontitis in patients with ESRD.

摘要

单核细胞趋化蛋白 1 (MCP-1) 在牙周炎的发展中起重要作用。本次调查的目的是评估MCP-1终末期肾病（ESRD）患者慢性牙周炎的基因多态性。本研究包括 150 名患有慢性牙周炎 (CP) 的 ESRD 患者、100 名没有 CP 的患者和 190 名健康对照者。所有参与者的基因组 DNA 通过聚合酶链反应 - 限制性片段长度多态性 (PCR-RFLP) 测定针对 –2518 (A/G) 多态性进行基因分型。在 ESRD 和 CP 患者与对照组之间的基因型和等位基因频率上观察到显着差异。患者的 G 等位基因频率显着高于对照组，优势比为 1.77 (95 % CI 1.2–2.5)，p = 0.0014。对于 GG 基因型，OR 为 3.63（95% CI 1.5–8.76），p = 0.041。在没有 CP 的 ESRD 患者和对照受试者之间未观察到多态性分布的显着差异。比较MCP-1基因多态性分布在终末期肾病各种原发病的终末期肾病患者中没有表现出任何显着差异。比较亚组之间的平均 MCP-1 血清水平。有 CP 的 ESRD 患者 (582 ± 112 pg/ml) 显着高于没有 CP 的患者 (309 ± 103 pg/ml) 和对照 (265 ± 85 pg/ml)。我们的研究结果表明，  MCP-1 -2518 A/G 多态性可能是 ESRD 患者发生慢性牙周炎的新危险因素。