Abstract For green synthesis in organic phases, the catalysis of lipases needs to be further improved. Here a strategy is to simulate the activation of lipases at water/oil interface by immobilizing lipase on an available carrier material, which can replace the water at the interface to stabilize enzyme against denaturation from organic phase. The carrier is selected as cotton fiber on which Pseudomonas cepacia lipase is immobilized by physical adsorption in a column glass bottle to form a simple bioreactor together with the bottle. In synthesis of fragrance ester hexyl acetate via transesterification of hexanol with vinyl acetate, the bioreactor exhibits a 3-fold increase in the ability to transform substrate, relative to native lipase in terms of the initial period of reaction at 37°C and 160 rpm. And also the bioreactor is very stable in catalysis for that it has an extra long half life t1/2 = 636 h, calculated from the decrease degree of molar conversions in six times of 6-hour transesterifications. More interestingly, the bioreactor behaves excellent activity at room temperature and in a static state, and can transform nearly 100% hexanol after 48 h. All these indicate that the bioreactor has great potential for industrial application.

中文翻译：

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一种用于有效合成乙酸己酯的简单室温静态生物反应器

摘要 为了实现有机相的绿色合成，脂肪酶的催化作用有待进一步提高。这里的一种策略是通过将脂肪酶固定在可用的载体材料上来模拟水/油界面处脂肪酶的活化，该载体材料可以取代界面处的水以稳定酶免受有机相的变性。载体选用棉纤维，通过物理吸附将洋葱假单胞菌脂肪酶固定在柱状玻璃瓶中，与瓶一起组成简易生物反应器。在通过己醇与乙酸乙烯酯的酯交换合成乙酸己酯芳香酯时，在 37°C 和 160 rpm 的反应初始阶段，生物反应器的底物转化能力比天然脂肪酶增加了 3 倍。并且生物反应器在催化方面非常稳定，因为它具有超长的半衰期 t1/2 = 636 小时，这是根据 6 次 6 小时酯交换反应中摩尔转化率的降低程度计算的。更有趣的是，该生物反应器在室温和静止状态下表现出优异的活性，48 小时后几乎可以转化 100% 的己醇。这些都表明生物反应器具有巨大的工业应用潜力。