Two new antimicrobial peptides belonging to brevinin 1 (B1) and brevinin 2 (B2) families were identified from the skin secretion of Indosylvirana aurantiaca, an endemic frog of Western Ghats, India, through shotgun cloning. Antibacterial, antibiofilm and cytotoxic effects of these peptides were evaluated and compared with their C-terminally amidated forms. Both the amidated peptides (B1-NH₂ and B2-NH₂) showed significantly enhanced broad-spectrum activities against Gram-positive and Gram-negative bacteria and the latter was found to have potent biofilm inhibition properties against Klebsiella pneumoniae over other peptides. Both forms of B2 were less cytotoxic against human red blood cells (hRBC) and peripheral blood mononuclear cell (PBMC). Hence, B2 and its amidated analog have good therapeutic value and these peptides could be considered as potential lead molecules for further development.

中文翻译：

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从印度西高止山脉的一种地方性蛙种印度支那虫的皮肤分泌物中鉴定出两种新型肽及其类似物的功能特性

通过散弹枪克隆，从印度西高止山脉的一种地方性青蛙Indosylvirana aurantiaca的皮肤分泌物中鉴定出了两个属于brevinin 1（B1）和brevinin 2（B2）家族的新抗菌肽。对这些肽的抗菌，抗生物膜和细胞毒性作用进行了评估，并与它们的C末端酰胺化形式进行了比较。两种酰胺化肽（B1-NH ₂和B2-NH ₂）均显示出对革兰氏阳性和革兰氏阴性细菌的广谱活性显着增强，并且发现后者对肺炎克雷伯菌具有有效的生物膜抑制作用。胜过其他肽。两种形式的B2对人红细胞（hRBC）和外周血单核细胞（PBMC）的细胞毒性都较小。因此，B2及其酰胺化类似物具有良好的治疗价值，这些肽可被视为潜在的进一步开发的先导分子。