Using next-generation sequencing, we detected a novel variant of hop stunt viroid (HSVd) in grapevine ‘Chenin blanc’ (Vitis vinifera L.) and a new viroid species in ‘Nachubearmarie’ (Vitis labrusca L. × V. vinifera). The HSVd variant termed HSVd-CB has 296 nucleotides with up to 82% sequence identity with other HSVd variants such as HSVd-AP1 (Genbank accession EF523826). Many nucleotide changes, deletions, and insertions were sporadically found in HSVd-CB relative to HSVd-AP1 in the pathogenic and variable domains. Although several variations were also found in the central domain, few variations were found in the terminal left and right domains, including no variations in the terminal conserved hairpin. The new viroid, tentatively termed Japanese grapevine viroid (JGVd), has 367 nucleotides and has genetic features characteristic of the genus Apscaviroid. JGVd shared the highest nucleotide sequence identity (68%) with a persimmon latent viroid (PLVd) in its overall genome. However, the JGVd sequence shows chimerism with the partial genomes of other apscaviroids from apple, grapevine, and citrus. Phylogenetic analyses showed that only HSVd-CB formed a distinct branch from the cluster of the other HSVd variants and JGVd and PLVd formed a distinct branch from all other grapevine-infecting apscaviroids.

使用下一代测序技术，我们在葡萄“ Chenin blanc”（葡萄属）中发现了一种新型的跳特技类病毒（HSVd），在“ Nachubearmarie”（葡萄属中出现了一种新的类病毒）（Vitis labrusca L.×  V. vinifera））。称为HSVd-CB的HSVd变体具有296个核苷酸，与其他HSVd变体（例如HSVd-AP1，Genbank登录号EF523826）的序列同一性高达82％。相对于HSVd-AP1在致病性和可变域中，在HSVd-CB中偶尔发现了许多核苷酸变化，缺失和插入。尽管在中央域中也发现了一些变异，但是在末端的左域和右域中几乎没有发现变异，包括末端保守发夹中没有变异。新的类病毒，暂称为日本葡萄类病毒（JGVd），具有367个核苷酸，并具有Apscaviroid属的遗传特征。。JGVd在其整个基因组中与柿子潜伏类病毒（PLVd）共享最高的核苷酸序列同一性（68％）。但是，JGVd序列与来自苹果，葡萄和柑橘的其他翼龙类的部分基因组显示出嵌合现象。系统发育分析表明，只有HSVd-CB与其他HSVd变体的簇形成一个明显的分支，而JGVd和PLVd与所有其他感染葡萄的拟猿猴形成一个明显的分支。