Abstract—We studied the effect of chronic neuropathic pain on the state of microglia and neurogenesis in the hippocampus in old mice. Neuropathic pain was induced by imposing three ligatures on the sciatic nerve of the right hind limb of animals. Testing behavior showed the presence of impaired working memory and locomotor activity in animals with neuropathic pain both in 1 week and 3 weeks after surgery. Behavioral disorders were accompanied by a decrease in hippocampal neurogenesis, as well as an increase in the expression of microglial markers Iba-1 and CD86 in the hippocampus of animals with a ligated sciatic nerve. In addition, the induction of neuropathic pain led to a change in the expression of astroglial markers S100β and GFAP in the hippocampus. The findings suggest that behavioral changes in neuropathic pain are accompanied by changes in the activity of microglia and astroglia, which leads to disruption of neurogenesis and a decrease in cognitive functions.

中文翻译：

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神经性疼痛对老年动物胶质细胞状态和海马神经发生的影响

摘要—我们研究了慢性神经性疼痛对老年小鼠海马小胶质细胞状态和神经发生的影响。通过在动物右后肢的坐骨神经上施加三个结扎来诱发神经性疼痛。测试行为表明，在术后1周和3周内，患有神经性疼痛的动物均存在工作记忆和运动能力受损。行为障碍伴有坐骨神经结扎动物海马中海马神经发生的减少，以及小胶质细胞标志物Iba-1和CD86的表达增加。另外，神经性疼痛的诱导导致海马中星形胶质标记物S100β和GFAP表达的变化。