This study was designed to identify several key genes and their functions in preventing or ameliorating intestinal ischemia–reperfusion (IR) injury, which could provide rationale for further exploring the regulatory mechanisms or clinical treatment for intestinal IR injury. The microarray GSE37013 of human intestinal IR injury was downloaded from Gene Expression Omnibus database. The differentially expressed genes (DEGs) with changes of reperfusion time were screened using Short Time-series Expression Miner, followed by function enrichment analysis, protein–protein interaction (PPI) network, and module construction. Subsequently, the key DEGs were identified with VEEN analysis based on the significant results of function enrichment analysis and PPI module. Finally, the gene–drug interactions were predicted using DGIdb 2.0. The DEGs of intestinal IR injury were significantly divided into three clusters with changes of reperfusion time. The genes in the three clusters were mainly enriched in transmembrane transport, defense responses, and cellular component assembly related pathways, respectively. There were 121 nodes and 281 interactions in PPI network, including one significant submodule. Protein tyrosine phosphatase, receptor type C (PTPRC) was a hub code both in PPI network and in submodule. A total of eight key DEGs were identified but only PTPRC was predicted to be interacted with eight drugs, such as infliximab. Totally, eight key genes associated with intestinal IR were identified; PTPRC especially was the most prominent potential drug target. These findings provided several potential therapeutic targets or potential breakthrough area in the study of intestinal IR injury.

中文翻译：

---

蛋白酪氨酸磷酸酶 C 型受体 (CD45) 在肠道缺血再灌注损伤中的潜在作用。

本研究旨在确定几个关键基因及其在预防或改善肠道缺血再灌注（IR）损伤中的功能，为进一步探索肠道 IR 损伤的调控机制或临床治疗提供理论依据。从基因表达综合数据库下载人肠道IR损伤微阵列GSE37013。使用Short Time-series Expression Miner筛选出随再灌注时间变化的差异表达基因（DEGs），然后进行功能富集分析、蛋白质-蛋白质相互作用（PPI）网络和模块构建。随后，基于功能富集分析和PPI模块的显着结果，通过VEEN分析确定了关键DEG。最后，使用 DGIdb 2.0 预测基因-药物相互作用。肠IR损伤的DEGs随着再灌注时间的变化显着分为三个簇。三个簇中的基因分别主要富集于跨膜转运、防御反应和细胞成分组装相关通路。PPI网络中有121个节点和281个交互，包括一个重要的子模块。蛋白酪氨酸磷酸酶，C 型受体 (PTPRC) 是 PPI 网络和子模块中的枢纽代码。总共确定了八个关键的 DEG，但预计只有 PTPRC 与八种药物相互作用，例如英夫利昔单抗。共鉴定出8个与肠道IR相关的关键基因；PTPRC 尤其是最突出的潜在药物靶点。