Energy‐metabolism oscillations (EMO) are ultradian biological rhythms observed in in aerobic chemostat cultures of Saccharomyces cerevisiae. EMO regulates energy metabolism such as glucose, carbohydrate storage, O₂ uptake, and CO₂ production. PSK1 is a nutrient responsive protein kinase involved in regulation of glucose metabolism, sensory response to light, oxygen, and redox state. The aim of this investigation was to assess the function of PSK1 in regulation of EMO. The mRNA levels of PSK1 fluctuated in concert with EMO, and deletion of PSK1 resulted in unstable EMO with disappearance of the fluctuations and reduced amplitude, compared with the wild type. Furthermore, the mutant PSK1Δ showed downregulation of the synthesis and breakdown of glycogen with resultant decrease in glucose concentrations. The redox state represented by NADH also decreased in PSK1Δ compared with the wild type. These data suggest that PSK1 plays an important role in the regulation of energy metabolism and stabilizes ultradian biological rhythms. These results enhance our understanding of the mechanisms of biorhythms in the budding yeast.

中文翻译：

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PSK1协调酿酒酵母中的葡萄糖代谢和利用，并调节能量代谢振荡。

能量代谢振荡（EMO）是在酿酒酵母的有氧化学恒温器培养物中观察到的超生物节律。EMO调节能量代谢，例如葡萄糖，碳水化合物的储存，O _2的吸收和CO _2的产生。PSK1是一种营养响应性蛋白激酶，参与葡萄糖代谢的调节，对光，氧和氧化还原状态的感觉响应。这项研究的目的是评估PSK1在EMO调节中的功能。与野生型相比，PSK1的mRNA水平与EMO一致波动，而PSK1的缺失导致EMO不稳定，波动消失，振幅降低。此外，突变体PSK1Δ显示出糖原合成和糖原的下调，导致葡萄糖浓度降低。与野生型相比，NADH代表的氧化还原状态在PSK1Δ中也降低。这些数据表明，PSK1在能量代谢的调节中起着重要的作用，并稳定了超diandian生物节律。这些结果增强了我们对发芽酵母中生物节律机制的理解。