This study evaluated the effect of aging methods on the bond strength of etch-and-rinse adhesive systems to dentin cavities. Eighty bovine incisors were used to prepare dentin cavities, which were bonded with different adhesive systems [Adper Scotchbond Multipurpose (SBMP), and Adper Single Bond 2 (SB)], and restored with composite resin. The specimens were stored in water for 24 h, and subjected to each aging method (n = 10): control group (not exposed to additional aging), thermal cycling (TC) (10,000 cycles; at temperatures of 5, 37, and 55 °C), mechanical loading (ML) (100,000 cycles; Hz; 60 N load), and TC + ML. The push-out bond strength test was performed using a universal testing machine. Failure modes were evaluated by scanning electron microscopy. Data were analyzed by two-way ANOVA and Tukey’s test (α = 0.05). For SBMP, only TC + ML decreased the bond strength compared to control group. For SB, all aging methods decreased similarly the bond strength compared to control group. A high number of adhesive failures were observed. Therefore, SB was sensitive to all aging methods, whilst only TC + ML was able to decrease the bond strength for SBMP.

中文翻译：

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时效方法对粘合剂体系对牙本质腔的推出粘结强度的影响

这项研究评估了老化方法对蚀刻和漂洗粘合剂系统与牙本质腔的粘合强度的影响。用八十个牛切牙准备牙本质腔，用不同的粘合剂系统粘合[Adper Scotchbond Multipurpose（SBMP）和Adper Single Bond 2（SB）]，并用复合树脂修复。将标本在水中保存24小时，然后采用每种老化方法（n = 10）：对照组（不进行额外老化），热循环（TC）（10,000个循环；在5、37和55的温度下） °C），机械负载（ML）（100,000个周期; Hz; 60 N负载）和TC + ML。使用通用测试机进行推出粘合强度测试。通过扫描电子显微镜评估失效模式。通过双向方差分析和Tukey检验分析数据（α= 0.05）。对于SBMP，与对照组相比，只有TC + ML降低了结合强度。对于SB，与对照组相比，所有老化方法均会类似地降低粘结强度。观察到大量的粘合失败。因此，SB对所有老化方法都很敏感，而只有TC + ML才能降低SBMP的粘结强度。