Arabidopsis hairy roots were used to produce human gastric lipase. When treated with 2,4-D, the hairy roots developed into thick organs that produced more protein than untreated roots. This was first assessed using green fluorescent protein-producing root lines from which the protein diffused into the culture medium. When growing hairy roots which express the human gastric lipase gene, very little lipase was found in the medium. Incubating the roots in a low pH buffer resulted in lipase diffusion into the buffer, avoiding the need for grinding. The activity of the enzyme on 4-methylumbellireryl-oleate and on tributyrin was determined. Approximately 6000 units of enzyme were recovered per gram of root. The enzyme was also extracted from freeze-dried roots before and after a 2-month storage period at room temperature. This work demonstrates the relevance of Arabidopsis hairy roots for the production of human gastric lipase.

拟南芥毛状根用于产生人胃脂肪酶。当用2,4-D处理时，毛状根会发展成较厚的器官，比未处理的根产生更多的蛋白质。首先使用产生绿色荧光蛋白的根系对其进行评估，蛋白质从该根系扩散到培养基中。当生长表达人胃脂肪酶基因的毛状根时，在培养基中几乎没有发现脂肪酶。在低pH缓冲液中孵育根导致脂肪酶扩散到缓冲液中，从而无需研磨。测定了该酶对4-甲基伞形基油酸酯和三丁酸甘油酯的活性。每克根大约可回收6000个单位的酶。在室温下保存2个月之前和之后，还从冻干的根中提取酶。这项工作证明了拟南芥毛状根，用于生产人胃脂肪酶。