In this study, we aimed to verify the effect of nutritional factors on the accretion of secondary metabolites in the adventitious root (AR) cultures of Malaysian ginseng (Eurycoma longifolia Jack) grown in small-scale bioreactors. AR were induced from leaf explants and cultured in different types of media including Murashige and Skoog (MS) medium, Driver Kuniyuki Walnut (DKW) medium, Gamborg’s B5 medium, Woody Plant Medium (WPM), and ¾ MS medium. Among these media, the MS and Gamborg’s B5 media induced lateral root development from initial inoculum, which accounted for the increase in AR biomass accretion. By contrast, the DKW and WPM media did not induce lateral root formation from the cultured explants. The ¾ MS medium was optimal for the growth of AR and accretion of secondary metabolites, after 7 weeks of culture, the biomass of AR increased by 8.6-fold in ¾ MS medium, and the total phenolic and flavonoid contents reached 5.23 and 2 mg g⁻¹ of tissue dry weight, respectively. Analysis of mineral elements in the spent medium revealed that ¾ MS medium was most suitable for nutrient supply to developing AR. LC–MS analysis showed the accretion of eurycomanone, a therapeutically useful metabolite, in the AR of Malaysian ginseng.

中文翻译：

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营养因素对马来西亚人参不定根培养物中次生代谢产物积累的影响

在这项研究中，我们旨在验证营养因素对马来西亚人参（Eurycoma longifolia）不定根（AR）培养物中次生代谢产物积累的影响。杰克（Jack）生长在小型生物反应器中。从叶外植体诱导AR，并在不同类型的培养基中培养，包括Murashige和Skoog（MS）培养基，Driver Kuniyuki Walnut（DKW）培养基，Gamborg's B5培养基，Woody Plant培养基（WPM）和¾MS培养基。在这些培养基中，MS和Gamborg的B5培养基从最初的接种物诱导了侧根发育，这解释了AR生物量的增加。相比之下，DKW和WPM培养基不会诱导培养的外植体形成侧根。培养7周后，¾MS培养基最适合AR的生长和次级代谢产物的积累，在¾MS培养基中AR的生物量增加了8.6倍，酚和类黄酮的总含量分别达到5.23和2 mg g ^-1组织干重分别。对用过的培养基中的矿质元素进行分析后发现，¾MS培养基最适合向发育中的AR提供营养。LC-MS分析表明，在马来西亚人参的AR中有治疗上有用的代谢产物eurycomanone的积聚。