Objective

This study aimed to investigate the role of zinc sulphate in immune regulation in Artemisia annua pollen-challenged P815 mastocytoma cells.

Methods

P815 mastocytoma cells were treated with various concentrations of zinc sulphate and Artemisia annua pollen. Cell proliferation was measured using the Cell Counting Kit-8. The amount of ST2 and p38 in the cells were measured using Western blotting. The level of interleukins (IL)-33 in the supernatant was determined using the enzyme-linked immunosorbent assay. The levels of IL-2, IL-4, IL-5, interferon-γ, and tumor necrosis factor were measured using the cytometric bead array.

Results

Artemisia annua

pollen at a concentration >0.001 µg/mL induced allergic response in the P815 mastocytoma cells. Expressions of IL-33, IL-4, ST2, and p38 increased along with higher concentrations of Artemisia annua pollen. Zinc sulphate of 50–200 µmol/L promoted the proliferation of P815 mastocytoma cells. Zinc sulphate attenuated the upregulation of IL-33, IL-4, ST2, and p38 caused by Artemisia annua pollen.

Conclusion

Zinc sulphate can promote the proliferation of P815 mastocytoma cells. It can also attenuate allergic response in the P815 mastocytoma cells induced by Artemisia annua pollen, which might provide a new treatment method for allergic diseases.

中文翻译：

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硫酸锌在青蒿花粉攻击的P815肥大细胞瘤细胞中的免疫调节作用。

目的

这项研究旨在调查硫酸锌在青蒿花粉攻击的P815肥大细胞瘤细胞中的免疫调节作用。

方法

用不同浓度的硫酸锌和青蒿花粉处理P815肥大细胞瘤细胞。使用Cell Counting Kit-8测量细胞增殖。使用蛋白质印迹法测量细胞中ST2和p38的量。使用酶联免疫吸附测定法测定上清液中白介素（IL）-33的水平。使用细胞计数珠阵列测量IL-2，IL-4，IL-5，干扰素-γ和肿瘤坏死因子的水平。

结果

青蒿

P815肥大细胞瘤细胞中浓度大于0.001 µg / mL的花粉诱导过敏反应。IL-33，IL-4，ST2和p38的表达随着青蒿花粉浓度的升高而增加。50–200 µmol / L的硫酸锌促进P815肥大细胞瘤细胞的增殖。硫酸锌减弱了由青蒿花粉引起的IL-33，IL-4，ST2和p38的上调。

结论

硫酸锌可促进P815肥大细胞瘤细胞的增殖。它也可以减轻青蒿花粉诱导的P815肥大细胞瘤细胞的过敏反应，这可能为过敏性疾病的治疗提供一种新的方法。