ABSTRACT

Background: During periodontal inflammation, bacteria induces chemokine expression and migration of various inflammatory cells. The aim of the study was to learn if periodontal treatment alters salivary concentrations of macrophage activation-related chemokines and if such alterations correlate with abundance of periodontitis-associated bacteria.

Methods: Twenty-five patients with periodontitis completed the study (NCT02913248 at clinicaltrials.gov). Periodontal parameters and stimulated saliva samples were obtained at baseline and 2, 6 and 12 weeks after non-surgical periodontal treatment. Salivary concentrations of monocyte chemoattractant proteins (MCP-1-4), macrophage-derived chemokine (MDC), macrophage migration inhibitory factor (MIF), monokine induced by interferon-gamma (MIG), macrophage inflammatory protein (MIP-1α) and interferon-inducible protein (IP-10) were quantified using the Luminex® xMAP™ technique and abundance of bacteria was quantified using next-generation sequencing.

Results: The treatment improved all periodontal parameters and caused an increase in the concentrations of MCP-2, MDC and MIP-1α at week 12 compared to baseline, week 2 and week 6, respectively. Salivary concentrations of MCP-1-2, MDC, MIG, MIP-1α and IP-10 correlated with the abundance of specific periodontitis-associated bacteria.

Conclusions: Periodontal treatment impacts salivary concentrations of MCP-2, MDC and MIP-1α, which correlate with the abundance of specific periodontitis-associated bacteria. This indicates that these chemokines reflect periodontal status and possess potential in illustrating a response to treatment.

 抽象的

背景：在牙周炎症过程中，细菌诱导趋化因子的表达和各种炎症细胞的迁移。该研究的目的是了解牙周治疗是否会改变巨噬细胞活化相关趋化因子的唾液浓度，以及这种改变是否与牙周炎相关细菌的丰度相关。

方法： 25 名牙周炎患者完成了研究（NCT02913248，网址：clinicaltrials.gov）。在基线以及非手术牙周治疗后 2、6 和 12 周获得牙周参数和刺激唾液样本。单核细胞趋化蛋白（MCP-1-4）、巨噬细胞衍生趋化因子（MDC）、巨噬细胞迁移抑制因子（MIF）、干扰素-γ诱导的单核因子（MIG）、巨噬细胞炎症蛋白（MIP-1α）和干扰素的唾液浓度-使用 Luminex® xMAP™ 技术对诱导蛋白 (IP-10) 进行定量，并使用新一代测序对细菌丰度进行定量。

结果：与基线、第 2 周和第 6 周相比，治疗改善了所有牙周参数，并导致第 12 周时 MCP-2、MDC 和 MIP-1α 的浓度分别增加。 MCP-1-2、MDC、MIG、MIP-1α 和 IP-10 的唾液浓度与特定牙周炎相关细菌的丰度相关。

结论：牙周治疗会影响 MCP-2、MDC 和 MIP-1α 的唾液浓度，这些浓度与特定牙周炎相关细菌的丰度相关。这表明这些趋化因子反映了牙周状态并具有说明治疗反应的潜力。