Circular RNAs (cicRNAs) have been identified to play pivotal roles in several cancer types. However, functions of circRNA in malignant melanoma are poor defined. Our current study demonstrated that human circMYC was obviously upregulated in human melanoma tissue. Furthermore, circMYC promoted the proliferation of human melanoma cells and Mel-CV cells. The expression of circMYC can repress Mel-CV cell glycolysis and LDHA activities in the in vitro glycolysis and lactate production evaluations. circMYC directly bound to miR-1236 as a molecular sponge that targeting miR-1236 in Mel-CV cells via bioinformatics analysis, pull-down assay, and luciferase reporter assays. Our present study revealed that 3′ UTR of LDHA acted as a target of miR-1236 using Mel-CV cells. Based on our findings, c-MYC-SRSF1 axis may regulate the production of circMYC. Overall, these results elucidate potential effects of circMYC in melanoma development and provide a promising biomarker for melanoma diagnosis.

中文翻译：

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CircMYC调节黑素瘤的糖酵解和细胞增殖

环状RNA（cicRNA）已被确定在几种癌症类型中起关键作用。但是，circRNA在恶性黑色素瘤中的功能尚不清楚。我们目前的研究表明，人circMYC在人黑素瘤组织中明显上调。此外，circMYC促进了人黑素瘤细胞和Mel-CV细胞的增殖。circMYC的表达可以在体外糖酵解和乳酸产生评估中抑制Mel-CV细胞的糖酵解和LDHA活性。circMYC作为分子海绵直接与miR-1236结合，通过生物信息学分析，下拉测定法和萤光素酶报告基因测定法靶向Mel-CV细胞中的miR-1236。我们目前的研究表明，使用Mel-CV细胞，LDHA的3'UTR充当miR-1236的靶标。根据我们的发现，c-MYC-SRSF1轴可能会调节circMYC的产生。总体，