Metroxylon sagu Rottb. or locally known as sago palm is a tropical starch crop grown for starch production in commercial plantations in Malaysia, especially in Sarawak, East Malaysia. This plant species accumulate the highest amount of edible starch compared to other starch-producing crops. However, the non-trunking phenomenon has been observed to be one of the major issues restricting the yield of sago palm starch. In this study, proteomics approach was utilised to discover differences between trunking and non-trunking proteomes in sago palm leaf tissues. Total protein from 16 years old trunking and non-trunking sago palm leaves from deep peat area were extracted with PEG fractionation extraction method and subjected to two-dimensional gel electrophoresis (2D PAGE). Differential protein spots were subjected to MALDI-ToF/ToF MS/MS. Proteomic analysis has identified 34 differentially expressed proteins between trunking and non-trunking sago samples. From these protein spots, all 19 proteins representing different enzymes and proteins have significantly increased in abundance in non-trunking sago plant when subjected to mass spectrometry. The identified proteins mostly function in metabolic pathways including photosynthesis, tricarboxylic acid cycle, glycolysis, carbon utilization and oxidative stress. The current study indicated that the several proteins identified through differentially expressed proteome contributed to physical differences in trunking and non-trunking sago palm.

中文翻译：

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西米棕榈蛋白的蛋白质组学，旨在鉴定耐胁迫品种中的蛋白质。

甲霜灵Rottb。或当地称为西米棕榈叶的一种热带淀粉作物，是在马来西亚，尤其是在东马来西亚沙捞越州的商业种植园种植淀粉生产的。与其他生产淀粉的农作物相比，这种植物积累的食用淀粉量最高。然而，已经观察到非结块现象是限制西米棕榈淀粉产量的主要问题之一。在这项研究中，蛋白质组学方法被用于发现西米棕榈叶组织中树干和非树干蛋白质组之间的差异。用PEG分级提取法提取了16岁树干和深层泥炭地非西米花西棕榈叶的总蛋白，并进行了二维凝胶电泳（2D PAGE）。对差异蛋白斑点进行MALDI-ToF / ToF MS / MS分析。蛋白质组学分析已经鉴定出在树干和非树干西米样品之间存在34种差异表达的蛋白质。从这些蛋白质斑点中，代表质谱图的所有19种代表不同酶和蛋白质的蛋白质在非矮生西米植物中的丰度都显着增加。鉴定出的蛋白质主要在代谢途径中起作用，包括光合作用，三羧酸循环，糖酵解，碳利用和氧化应激。当前的研究表明，通过差异表达的蛋白质组鉴定出的几种蛋白质促成了西葫芦和非西葫芦的物理差异。接受质谱分析的所有19种代表不同酶和蛋白质的蛋白质在非矮生西米植物中的丰度都显着增加。鉴定出的蛋白质主要在代谢途径中起作用，包括光合作用，三羧酸循环，糖酵解，碳利用和氧化应激。当前的研究表明，通过差异表达的蛋白质组鉴定出的几种蛋白质促成了西葫芦和非西葫芦的物理差异。进行质谱分析时，代表非酶和西米植物的所有19种代表不同酶和蛋白质的蛋白质的丰度都显着增加。鉴定出的蛋白质主要在代谢途径中起作用，包括光合作用，三羧酸循环，糖酵解，碳利用和氧化应激。当前的研究表明，通过差异表达的蛋白质组鉴定出的几种蛋白质促成了西葫芦和非西葫芦的物理差异。