In this article, we describe an in‐house polymerase chain reaction‐sequence specific priming (PCR‐SSP) assay designed for undergraduate medical students as part of the experimental pathogen biology and immunology (EPBI) course. It screens human leukocyte antigen (HLA)‐DR2 allotype from genomic DNA samples using a rapid and single‐tube PCR technique, yielding definitive typing result without conventional post‐amplification step like probing or Sanger sequencing. This laboratory exercise offers the undergraduate medical students an opportunity to learn about current molecular biology techniques in HLA genotyping with limited effort and cost, in addition to a better understanding of concepts presented in the classroom lectures. Upon completing this experiment module, the students show statistically significant improvement in several key indexes, such as the knowledge about the mainstream HLA DNA typing techniques, awareness of the relevance of this knowledge for their future scientific research, immunogenetics‐related basic laboratory skills they acquire, and interest and desire for mastering this assay (all p < .05). This easy to implement set of experiments is composed of a two‐session lab module occupying eight teaching hours, and has been run successfully in our laboratory.

中文翻译：

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本科医学生免疫学课程使用聚合酶链反应-序列特异性启动技术从生殖系DNA中快速筛选HLA-DR2同种异型的实验室实践

在本文中，我们描述了一种为本科医学生设计的内部聚合酶链反应序列特异性启动 (PCR-SSP) 检测，作为实验病原体生物学和免疫学 (EPBI) 课程的一部分。它使用快速单管 PCR 技术从基因组 DNA 样本中筛选人类白细胞抗原 (HLA)-DR2 同种异型，无需常规的扩增后步骤（如探测或 Sanger 测序）即可产生明确的分型结果。除了更好地理解课堂讲座中提出的概念之外，该实验室练习还为本科医学生提供了一个机会，以有限的精力和成本了解 HLA 基因分型的当前分子生物学技术。完成本实验模块后，学生在几个关键指标上表现出统计学上的显着改善，例如关于主流 HLA DNA 分型技术的知识、对这些知识与他们未来科学研究的相关性的认识、他们获得的与免疫遗传学相关的基本实验室技能，以及掌握该检测方法的兴趣和愿望（所有 p < .05）。这套易于实施的实验由一个两节课的实验模块组成，占用了 8 个教学小时，并已在我们实验室成功运行。