BACKGROUND A new type of deep eutectic solvent based on three components using phosphate salts has been synthesized, characterized, and applied in the extraction of eight organothiophosphate pesticides from honey samples. In the present study, the deep eutectic solvent was prepared from phosphocholine choline chloride as a hydrogen bond acceptor and dichloroacetic acid and decanoic acid as hydrogen bond donors. The method consisted of two steps in which initially the analytes were extracted from the samples into a water-miscible organic solvent. In the second step, the extracted phase was mixed with the prepared deep eutectic solvent and the mixture was used in the following dispersive liquid-liquid microextraction method. RESULTS The method was validated under optimal conditions, and it was found that the method has low limits of detection (0.05-0.10 ng g-1 ) and quantification (0.19-0.36 ng g-1 ), good linearity (r2 ≥ 0.994), broad linearity (0.36-1000 ng g-1 ), and satisfactory repeatability (relative standard deviation ≤ 10% for intra- (n=6) and inter-day (n=4) precisions at a concentration of 2 ng g-1 of each analyte). CONCLUSION Finally, the proposed method was applied in different honey samples, and malathion was found at a concentration of 29 ng g-1 in one sample. This article is protected by copyright. All rights reserved.

中文翻译：

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基于氯化磷胆碱的三组分低共熔溶剂的合成与表征：在分散液液微萃取中测定有机硫代磷农药的应用

背景技术利用磷酸盐合成、表征了一种新型的三组分深共熔溶剂，并将其应用于从蜂蜜样品中提取八种有机硫代磷农药。在本研究中，以磷酸胆碱氯化胆碱为氢键受体，二氯乙酸和癸酸为氢键供体制备深共熔溶剂。该方法包括两个步骤，其中首先将分析物从样品中提取到与水混溶的有机溶剂中。第二步，将萃取相与制备好的低共熔溶剂混合，用于以下分散液液微萃取法。结果 在最佳条件下对该方法进行了验证，发现该方法的检出限较低（0. 05-0.10 ng g-1 ）和定量（0.19-0.36 ng g-1 ），良好的线性（r2 ≥ 0.994），宽线性（0.36-1000 ng g-1 ），以及令人满意的重复性（相对标准偏差≤ 10%）对于每种分析物浓度为 2 ng g-1 时的内 (n=6) 和日间 (n=4) 精度）。结论 最后，将所提出的方法应用于不同的蜂蜜样品，在一个样品中发现浓度为 29 ng g-1 的马拉硫磷。本文受版权保护。版权所有。在一个样品中发现浓度为 29 ng g-1 的马拉硫磷。本文受版权保护。版权所有。在一个样品中发现浓度为 29 ng g-1 的马拉硫磷。本文受版权保护。版权所有。