Antimicrobial resistance due to carbapenemase production in Enterobacteriaceae clinical isolates is a global threat. Klebsiella pneumoniae harboring the bla_KPC gene is one of the major concerns in hospital settings in Latin America.

The aim of this study was to characterize the antibiotic resistance mechanisms and to typify four carbapenem-resistant K. pneumoniae clinical isolates from the city of Manizales, Colombia.

We identified bla_KPC-3 in all four isolates by polymerase chain reaction and subsequent sequencing. The plasmid-mediated quinolone resistance genes qnrB19-like and aac(6′)Ib-cr; fosfomycin resistance gene fosA and an insertion sequence IS5-like in mgrB (colistin resistance) were also detected. Sequence types ST11 with capsular type wzi75, and ST258 with wzi154, were characterized. The bla_KPC-3 gene was mobilized in a 100-kb IncFIB conjugative plasmid with vagCD toxin–antitoxin system.

This work reports multiple resistance genes in bla_KPC-producing K. pneumoniae and the first occurrence of ST11 clinical isolates harboring bla_KPC-3 in Latin America.

肠杆菌科临床分离株产生碳青霉烯酶导致的抗菌素耐药性是一个全球性威胁。携带bla _KPC基因的肺炎克雷伯 菌是拉丁美洲医院环境中的主要问题之一。

本研究的目的是描述抗生素耐药机制，并对来自哥伦比亚马尼萨莱斯市的四种耐碳青霉烯类肺炎克雷伯菌临床分离株进行分类。

我们通过聚合酶链反应和随后的测序在所有四种分离物中鉴定了bla _{KPC-3 。}质粒介导的喹诺酮类耐药基因qnrB19-like和aac(6′)Ib-cr；还检测到磷霉素抗性基因fosA和mgrB中的插入序列 IS 5样（粘菌素抗性）。序列类型 ST11 与荚膜型wzi 75 和 ST258 与wzi 154 进行了表征。bla _KPC-3基因被移动到一个 100-kb IncFIB 结合质粒中，该质粒具有vagCD毒素-抗毒素系统。

这项工作报告了产生bla _KPC的肺炎克雷伯菌中的多个抗性基因，以及拉丁美洲首次出现含有bla _KPC-3的 ST11 临床分离株。