Eruca sativa action on the male reproductive system and fertility has not been precisely defined. In this study, the aim was to investigate the ameliorative activity of E. sativa aqueous extracts (ESAE) on reproductive toxicity associated with oxidative stress induced by bisphenol A (BPA). Wistar rats were used and divided into six groups of animals each; control (0.4 mL of corn oil/rat), ESAE at the higher dose (200 mg/kg), BPA [100 mg/kg, body weight (b.w.), perorally (p.o.)] alone, or in combination with varied doses of ESAE (50, 100, and 200 mg/kg, b.w, p.o.). The diverse doses were administrated orally for 30 consecutive days. The results showed that BPA-treatment produced a diminution of density, motility, and viability of sperm with disruption of spermatozoa morphology and fertilizing potential as well as testosterone, luteinizing hormone, and follicle stimulating hormone levels. These results were accompanied by testis and epididymis histological damages, which were shown by an induction of testicular dysfunction as seen with a lower number of Leydig-cells and spermatocytes as well as a reproductive stress which was modeled. The oxidative stress was measured by malondialdehyde production, thiol group (-SH) decline and antioxidant enzyme activities disturbance, in particular superoxide dismutase, catalase, and glutathione peroxidase in reproductive tissues. ESAE coadministration at the two lower doses improved all histological and biochemical parameter injuries. These finding suggested the ESAE ability to prevent the testicular damages in rats, which might be linked to functional-bioactive substances such as phenolic compounds with higher antioxidant capacity.

中文翻译：

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紫草叶子水提取物对双酚A引起的体内睾丸损伤的保护作用。

紫草对雄性生殖系统和生育力的作用尚未精确定义。在这项研究中，目的是研究紫花苜蓿的改良活性。水提取物（ESAE）对与双酚A（BPA）诱导的氧化应激相关的生殖毒性。使用Wistar大鼠，每组分为六组。对照（0.4 mL玉米油/大鼠），较高剂量的ESAE（200 mg / kg），BPA [100 mg / kg，体重（bw），经口（po）]单独使用，或与不同剂量的ESAE（50、100和200 mg / kg，体重，口服）。连续30天口服各种剂量。结果表明，双酚A处理会降低精子的密度，运动性和活力，从而破坏精子的形态和受精能力，并破坏睾丸激素，促黄体生成激素和刺激卵泡的激素水平。这些结果伴有睾丸和附睾组织学损伤，其表现为睾丸功能障碍的诱导，如较少的Leydig细胞和精母细胞以及模拟的生殖压力。通过丙二醛产生，巯基（-SH）下降和抗氧化酶活性扰动，特别是生殖组织中的超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶来测量氧化应激。两种较低剂量的ESAE共同给药改善了所有组织学和生化参数损伤。这些发现表明，ESAE具有预防大鼠睾丸损伤的能力，这可能与功能性生物活性物质（例如具有较高抗氧化能力的酚类化合物）有关。通过丙二醛产生，巯基（-SH）下降和抗氧化酶活性扰动，特别是生殖组织中的超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶来测量氧化应激。两种较低剂量的ESAE共同给药改善了所有组织学和生化参数损伤。这些发现表明，ESAE具有预防大鼠睾丸损伤的能力，这可能与功能性生物活性物质（例如具有较高抗氧化能力的酚类化合物）有关。通过丙二醛产生，巯基（-SH）下降和抗氧化酶活性扰动，特别是生殖组织中的超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶来测量氧化应激。两种较低剂量的ESAE共同给药改善了所有组织学和生化参数损伤。这些发现表明，ESAE具有预防大鼠睾丸损伤的能力，这可能与功能性生物活性物质（例如具有较高抗氧化能力的酚类化合物）有关。