DNA methylation is an epigenetic factor, which plays important roles in embryo and many other diseases development. This factor determines gene expression, and when half of them have CpG islands, DNA methylation and its enzyme effectors have been under the vast studies. Whole genome DNA demethylation is a crucial step of embryogenesis and also cell fate determination in embryos. Therefore, demethylation agents were used as a tool for dedifferentiation and transdifferentiation. Although many of these efforts have been successful, but using this method gave us a vast spectral cell type which is confusing. In this article, we briefly reviewed DNA methylation, and its role in embryogenesis and gene expression. In addition to that, we introduce studies that used this action as a direct method in induction of stem cells and cell fate decision.

DNA甲基化是一种表观遗传因子，在胚胎和许多其他疾病的发展中起着重要作用。这个因素决定了基因的表达，当其中一半具有CpG岛时，DNA甲基化及其酶效应子已被广泛研究。全基因组DNA去甲基化是胚胎发生以及胚胎中细胞命运确定的关键步骤。因此，脱甲基剂被用作去分化和反分化的工具。尽管这些努力中的许多已经成功，但是使用这种方法给我们带来了令人困惑的巨大光谱细胞类型。在本文中，我们简要回顾了DNA甲基化及其在胚胎发生和基因表达中的作用。除此之外，我们还介绍了使用该作用作为诱导干细胞和细胞命运决定的直接方法的研究。