We evaluated the effects of the number of years of restoration of vegetation on soil microbial community structure and biomass in degraded ecosystems. We investigated the microbial community structure by analyzing their phospholipid fatty acids then examined microbial biomass carbon and nitrogen by chloroform fumigation extraction of restoration soils over several years. The data were compared with those of highly degraded lands and native vegetation sites. The results show that the duration of vegetation on the sites substantially increased microbial biomass and shifted the microbial community structure even after only 4 years. However, microbial communities and biomass did not recover to the status of native vegetation even after 35 years of vegetation cover. A redundancy analysis and Pearson correlation analysis indicated that soil organic carbon, total nitrogen, available potassium, soil water content, silt content and soil hardness explained 98.4% of total variability in the microbial community composition. Soil organic carbon, total nitrogen, available potassium and soil water content were positively correlated with microbial community structure and biomass, whereas, soil hardness and silt content were negatively related to microbial community structure and biomass. This study provides new insights into microbial community structure and biomass that influence organic carbon, nitrogen, phosphorus and potassium accumulation, and clay content in soils at different stages of restoration.

我们评估了植被恢复年限对退化的生态系统中土壤微生物群落结构和生物量的影响。我们通过分析其磷脂脂肪酸来研究微生物群落结构，然后通过氯仿熏蒸法提取修复土壤中的微生物生物量碳和氮，历时数年。将数据与高度退化的土地和原生植被地点的数据进行了比较。结果表明，即使仅4年，这些地点的植被持续时间也大大增加了微生物的生物量，并改变了微生物群落结构。然而，即使经过35年的植被覆盖，微生物群落和生物量也无法恢复到天然植被的状态。冗余分析和Pearson相关分析表明，土壤有机碳，总氮，有效钾，土壤水分，淤泥含量和土壤硬度解释了微生物群落组成总变异的98.4％。土壤有机碳，总氮，速效钾和土壤水分与微生物群落结构和生物量呈正相关，而土壤硬度和淤泥含量与微生物群落结构和生物量呈负相关。这项研究为微生物群落结构和生物量提供了新的见解，这些微生物和生物量在恢复的不同阶段影响土壤中有机碳，氮，磷和钾的积累以及黏土含量。微生物群落组成总变异的4％。土壤有机碳，总氮，速效钾和土壤水分与微生物群落结构和生物量呈正相关，而土壤硬度和淤泥含量与微生物群落结构和生物量呈负相关。这项研究为微生物群落结构和生物量提供了新的见解，这些微生物和生物量在恢复的不同阶段影响土壤中有机碳，氮，磷和钾的积累以及黏土含量。微生物群落组成总变异性的4％。土壤有机碳，总氮，速效钾和土壤水分与微生物群落结构和生物量呈正相关，而土壤硬度和淤泥含量与微生物群落结构和生物量呈负相关。这项研究为微生物群落结构和生物量提供了新的见解，这些微生物和生物量在恢复的不同阶段影响土壤中有机碳，氮，磷和钾的积累以及黏土含量。土壤硬度和泥沙含量与微生物群落结构和生物量呈负相关。这项研究为微生物群落结构和生物量提供了新的见解，这些微生物和生物量在恢复的不同阶段影响土壤中有机碳，氮，磷和钾的积累以及黏土含量。土壤硬度和泥沙含量与微生物群落结构和生物量呈负相关。这项研究为微生物群落结构和生物量提供了新的见解，这些微生物和生物量在恢复的不同阶段影响土壤中有机碳，氮，磷和钾的积累以及黏土含量。