Detection of the fusion gene composed of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) is important for diagnosis of lung adenocarcinoma. We developed a simple PCR-based fluorometric method for detecting the EML4-ALK fusion using a fluorescent probe DNA and graphene oxide (GO). The fluorophore-labeled probe DNA complementary to the EML4-ALK fusion junction was hydrolyzed during PCR by the 5′ to 3′ exonuclease activity of the Taq DNA polymerase. The fluorophore released from the probe DNA retained fluorescence without adsorption onto GO. Our GO-based fluorometric system was able to detect a single cancer cell harboring the EML4-ALK fusion in 10⁶ wild-type cells.

中文翻译：

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基于氧化石墨烯的荧光法检测致癌EML4-ALK融合基因

检测由棘皮动物微管相关蛋白样4（EML4）和间变性淋巴瘤激酶（ALK）组成的融合基因对于诊断肺腺癌非常重要。我们开发了一种基于PCR的简单荧光方法，用于使用荧光探针DNA和氧化石墨烯（GO）检测EML4-ALK融合。PCR期间，Taq DNA聚合酶的5'至3'核酸外切酶活性水解了与EML4-ALK融合连接互补的荧光团标记探针DNA 。从探针DNA释放的荧光团保留了荧光而不吸附到GO上。我们基于GO的荧光测定系统能够检测到带有EML4-ALK的单个癌细胞融合在10 ^6个野生型细胞中。