Ex vivo fluorescence confocal microscopy (FCM) is an optical technology that provides fast H&E-like images of freshly excised tissues, and it has been mainly used for "real-time" pathological examination of dermatological malignancies. It has also shown to be a promising tool for fast pathological examination of prostatic tissues. We aim to create an atlas for FCM images of prostatic and periprostatic tissues to facilitate the interpretation of these images. Furthermore, we aimed to evaluate the learning curve of images interpretation of this new technology. Eighty fresh and unprepared biopsies obtained from radical prostatectomy specimens were evaluated using the FCM VivaScope® 2500 M-G4 (Mavig GmbH, Munich, Germany; Caliber I.D.; Rochester NY, USA) by two pathologists. Images of FCM with the corresponding H&E are illustrated to create the atlas. Furthermore, the two pathologists were asked to re-evaluate the 80 specimens after 90 days interval in order to assess the learning curve of images' interpretation of FCM. FCM was able to differentiate between different types of prostatic and periprostatic tissues including benign prostatic glands, benign prostatic hyperplasia, high-grade intraepithelial neoplasm, and prostatic adenocarcinoma. As regards the learning curve, FCM demonstrated a short learning curve. We created an atlas that can serve as the base for urologists and pathologists for learning and interpreting FCM images of prostatic and periprostatic tissues. Furthermore, FCM images is easily interpretable; however, further studies are required to explore the potential applications of this new technology in prostate cancer diagnosis and management.

中文翻译：

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离体荧光共聚焦显微镜：前列腺和前列腺组织图集和学习曲线评估。

离体荧光共聚焦显微镜（FCM）是一种光学技术，可为新鲜切除的组织提供快速的H＆E样图像，它已主要用于皮肤恶性肿瘤的“实时”病理检查。它也已被证明是前列腺组织快速病理检查的有前途的工具。我们旨在为前列腺和前列腺周围组织的FCM图像创建图集，以帮助解释这些图像。此外，我们旨在评估这种新技术的图像解释的学习曲线。由两位病理学家使用FCM 2500 M-G4（德国慕尼黑的马维格公司；口径ID；美国纽约州罗彻斯特）对从根治性前列腺切除术标本中获得的80份新鲜活检样品进行了评估。FCM的图像以及相应的H＆图示了E以创建图集。此外，还要求两位病理学家在90天间隔后重新评估80个标本，以评估图像对FCM解释的学习曲线。FCM能够区分不同类型的前列腺组织和前列腺组织，包括良性前列腺，良性前列腺增生，高级别上皮内肿瘤和前列腺腺癌。关于学习曲线，FCM显示出较短的学习曲线。我们创建了一个地图集，可作为泌尿科医生和病理学家学习和解释前列腺和前列腺周围组织的FCM图像的基础。此外，FCM图像很容易解释。然而，