The aim of this study was to estimate the level of genomic and phenotypic diversity as well as the genus and species position of bacterial strains isolated from root nodules of Lembotropis nigricans (family Fabaceae). The genomic diversity of studied L. nigricans nodule symbionts was examined by using BOX-PCR and AFLP (amplified fragment length polymorphism) fingerprinting techniques. To assign bacteria to the genus, numerical analysis of phenotypic features and comparative analysis of 16S rDNA sequences were performed. The comparative analysis of combined atpD, dnaK, gyrB, and rpoB gene sequences (multilocus sequence analysis, MLSA) was used to determine the most closely related species to the studied bacteria. Both BOX-PCR and AFLP techniques revealed a high level of genomic heterogeneity of L. nigricans nodulators. Among 33 studied bacteria, 32 genotypes were delineated by the AFLP method and 27 genotypes were identified by the BOX-PCR fingerprinting. The numerical analysis of 86 phenotypic characteristics of L. nigricans nodule isolates and reference rhizobia showed that studied bacteria belong to the genus Bradyrhizobium. Affiliation of L. nigricans nodule isolates to the genus Bradyrhizobium was supported by comparative analysis of 16S rDNA sequences and the concatenation of atpD, dnaK, gyrB, and rpoB gene sequences. MLSA indicated also that L. nigricans microsymbionts are members of Bradyrhizobium japonicum. L. nigricans root nodule symbionts are members of Bradyrhizobium japonicum and exhibit high phenotypic and genomic diversity important for their survival in soil.

中文翻译：

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从Genisteae部落黑腹蝗根瘤分离的根瘤菌菌株的表型特性的数值分析，基因组指纹图谱和多位点序列分析。

这项研究的目的是估计基因组和表型多样性的水平，以及从黑embo（根root科）的根瘤分离出的细菌菌株的属和种位置。通过使用BOX-PCR和AFLP（扩增的片段长度多态性）指纹图谱技术，检查了研究的黑麦草根瘤共生菌的基因组多样性。为了将细菌分配给该属，进行了表型特征的数值分析和16S rDNA序列的比较分析。组合的atpD，dnaK，gyrB和rpoB基因序列的比较分析（多基因座序列分析，MLSA）用于确定与所研究细菌最密切相关的物种。BOX-PCR和AFLP技术均显示了黑黑麦草结瘤菌的高水平基因组异质性。在研究的33种细菌中，通过AFLP方法描绘了32种基因型，并且通过BOX-PCR指纹图谱鉴定了27种基因型。对黑黑麦草根瘤菌和参考根瘤菌的86个表型特征进行了数值分析，结果表明所研究的细菌属于缓生根瘤菌属。对16S rDNA序列的比较分析以及atpD，dnaK，gyrB和rpoB基因序列的串联支持了黑麦草根瘤菌与缓生根瘤菌属的隶属关系。MLSA还指出，黑乳杆菌微共生菌是日本慢生根瘤菌的成员。黑麦草根瘤结节共生体是日本根瘤菌，表现出高表型和基因组多样性，对于它们在土壤中的存活很重要。对黑黑麦草根瘤菌和参考根瘤菌的86个表型特征进行了数值分析，结果表明所研究的细菌属于缓生根瘤菌属。对16S rDNA序列的比较分析以及atpD，dnaK，gyrB和rpoB基因序列的串联支持了黑麦草根瘤菌与缓生根瘤菌属的隶属关系。MLSA还指出，黑乳杆菌微共生菌是日本慢生根瘤菌的成员。黑麦草根瘤结节共生体是日本根瘤菌，表现出高表型和基因组多样性，对于它们在土壤中的存活很重要。对黑黑麦草根瘤菌和参考根瘤菌的86个表型特征进行了数值分析，结果表明所研究的细菌属于缓生根瘤菌属。对16S rDNA序列的比较分析以及atpD，dnaK，gyrB和rpoB基因序列的串联支持了黑麦草根瘤菌与缓生根瘤菌属的隶属关系。MLSA还指出，黑乳杆菌微共生菌是日本慢生根瘤菌的成员。黑麦草根瘤结节共生体是日本根瘤菌，表现出高表型和基因组多样性，对于它们在土壤中的存活很重要。通过比较分析16S rDNA序列以及atpD，dnaK，gyrB和rpoB基因序列的串联，支持将根瘤菌属的黑头根瘤菌分离出来。MLSA还指出，黑乳杆菌微共生菌是日本慢生根瘤菌的成员。黑麦草根瘤结节共生体是日本根瘤菌，表现出高表型和基因组多样性，对于它们在土壤中的存活很重要。通过比较分析16S rDNA序列以及atpD，dnaK，gyrB和rpoB基因序列的串联，支持将根瘤菌属的黑头根瘤菌分离出来。MLSA还指出，黑乳杆菌微共生菌是日本短枝根瘤菌的成员。黑麦草根瘤结节共生体是日本根瘤菌，表现出高表型和基因组多样性，对于它们在土壤中的存活很重要。