Malachite green (MG) is a banned fish drug, but is often found in various aquatic food products. In this study, Au nanoparticles (NPs) were incorporated with graphene oxide (GO) to fabricate Au NPs-GO hybrid films as surface enhanced Raman scattering (SERS) substrate for MG detection. The enhancement effect of the hybrid substrate for SERS signal of MG as influenced by GO concentration, size of Au NPs and pretreatment method for quartz sheets were evaluated. The optimal Au NPs-GO substrate was used to detect MG in fish muscle with SERS technology. The lowest detectable concentration for MG standard solution was 0.01 ng/mL, and the concentration of 0.25 ng/g could be detected in the muscle extracts of two different fish. SERS combined with Au NPs-GO hybrid substrate shows great potential for detection of trace amounts of MG in aquatic products.

中文翻译：

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以金纳米颗粒和氧化石墨烯杂化物为表面增强拉曼散射的底物超灵敏地检测鱼肉中的孔雀石绿

孔雀石绿（MG）是一种禁用的鱼药，但经常在各种水生食品中发现。在这项研究中，金纳米颗粒（NPs）与氧化石墨烯（GO）结合在一起，以制造金纳米颗粒-GO杂化膜作为用于MG检测的表面增强拉曼散射（SERS）衬底。评价了GO底物，Au NPs的大小和石英片的预处理方法对杂色底物对MGSERS信号的增强作用。最佳的金纳米颗粒-GO底物通过SERS技术用于检测鱼肌肉中的MG。MG标准溶液的最低可检测浓度为0.01 ng / mL，并且在两种不同鱼类的肌肉提取物中可检测到0.25 ng / g的浓度。SERS与Au NPs-GO杂化底物的结合显示出检测水产品中痕量MG的巨大潜力。