Blister blight is the most prevalent leaf disease of tea, an economical perennial crop. The disease is caused by a biotrophic fungus, Exobasidium vexans. The objective was set to study whether the combine over-expression of Solanum tuberosum class I chitinase gene and Vigna radiata defensin genes in transgenic tea would give more disease resistance compared to transgene over-expressed singly. Taking two constructs containing S. tuberosum class I chitinase (AF153195) and mung bean defensin (AY437639) gene respectively, three Agrobacterium-mediated transformation events were conducted with two individual constructs and combination of both the constructs. This is a first report about the preparation of transgenic tea by co-transformation for blister resistance. Transformation was successful in all three events. Comparative analysis showed a very interesting result. Minimum overall transformation efficiency was noticed in transformation of tea with pCAMBIA 1301-Chi (2.20%) followed by pBI121-Def (2.39%) and co-transformation with pCAMBIA 1301-Chi and pBI121-Def (3.41%). The fungal bioassay with E. vexans for the transformation event with pCAMBIA 1301-Chi gave the best disease resistance having minimum diameter (7.05 mm) of hypersensitivity zone followed by 15.05 mm in pBI121-Def and 18.14 mm in combination of pCAMBIA 1301-Chi and pBI121-Def.

中文翻译：

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农杆菌介导的茶对水疱病的抗性共转化研究

水泡枯萎病是茶的最普遍的叶病，一种经济的多年生作物。这种疾病是由一种营养丰富的真菌，即Exobasidium v​​exans引起的。目的是研究与单独过表达的转基因相比，转基因茶中马铃薯I类几丁质几丁质酶基因和紫花防御素基因的组合过表达是否会产生更多的抗病性。服用含有两种构建马铃薯类几丁质酶我（AF153195）和绿豆防御素（AY437639）基因分别，三根介导的转化事件是用两种单独的构建物以及两种构建物的组合进行的。这是有关通过共转化制备起泡性茶的转基因茶的首次报道。在所有三个事件中，转换都是成​​功的。比较分析显示了非常有趣的结果。在用pCAMBIA 1301-Chi（2.20％），然后是pBI121-Def（2.39％）以及与pCAMBIA 1301-Chi和pBI121-Def（3.41％）的共转化中，茶的最低总体转化效率被注意到。用p.VCAMans进行pCAMBIA 1301-Chi转化的真菌生物测定法具有最佳的抗病性，其超敏区的直径最小（7.05 mm），其次是pBI121-Def为15.05 mm，而pCAMBIA 1301-Chi和pCAMBIA 1301-Chi的组合为18.14 mm。 pBI121-Def。