This study investigates the biocontrol potential of Trichoderma asperellum mutants against Rhizoctonia solani, Alternaria alternata, and Fusarium oxysporum and growth promotion of Populus davidiana × P. alba var. pyramidalis (PdPap poplar) seedlings. A T-DNA insertion mutant library of T. asperellum was constructed using Agrobacterium tumefaciens-mediated transformation. Sixty-five positive transformants (T1–T65) were obtained. Growth rates of the mutants T39 and T45 were the same, 39.68% faster than the WT. In toxin tolerance tests, only T39 had greater tolerance to A. alternata fermentation broth than the WT, but mutant T45 had the same tolerance as the WT to all fermentation broths. Furthermore, T39 and T45 had a greater antagonistic ability than the WT strain against R. solani and A. alternata. The inhibition rate of the mutants T39 and T45 against A. alternata are 73.92% and 80.76%, respectively, and 63.51% and 63.74%, respectively. Furthermore, the three strains increased the activities of superoxide dismutases, peroxidase, catalase (CAT) and phenylalanine ammonia lyase (PAL) in PdPap seedling leaves. CAT and PAL activity in the PdPap seedling leaves was 11.25 and 5.50 times higher, respectively, in the presence of T39 than in the control group and 12 and 6.35 times higher, respectively, in the presence of T45 than in the control group. All three strains promoted seedling growth and the root and stem development, especially mutant T45. Mutants T39 and T45 reduced the incidence of pathogenic fungi in poplar and stimulated poplar seedling growth.

这项研究调查的生物防治潜力棘孢木霉对突变体水稻纹枯病菌，交链孢和尖孢镰刀菌促生长的山杨 × 银白杨变种。金字塔形（PdPap杨树）幼苗。利用根癌农杆菌介导的转化构建了曲霉菌的T-DNA插入突变体文库。获得了65个阳性转化子（T1-T65）。突变体T39和T45的生长速率相同，比WT快39.68％。在毒素耐受性测试中，只有T39对交链杆菌有更大的耐受性发酵液比野生型肉汤高，但突变体T45与野生型对所有发酵液的耐受性相同。此外，与WT菌株相比，T39和T45具有更大的拮抗R.solani和A. alternata的能力。突变体T39和T45对交链孢霉的抑制率分别是73.92％和80.76％，以及63.51％和63.74％。此外，这三个菌株增加了PdPap幼苗叶片中的超氧化物歧化酶，过氧化物酶，过氧化氢酶（CAT）和苯丙氨酸氨裂合酶（PAL）的活性。在存在T39的情况下，PdPap幼苗叶片中的CAT和PAL活性分别比对照组高11.25和5.50倍，在存在T45的情况下分别比对照组高12和6.35倍。这三种菌株均促进了幼苗的生长以及根和茎的发育，特别是突变体T45。T39和T45突变体减少了杨树中病原真菌的发生率，并刺激了杨树幼苗的生长。