Stacksbinder (available at https://ps.agr.ryukoku.ac.jp/stacksbinder/) is a web-based tool for summarizing the output of software Stacks, which is widely used to analyze restriction site-associated DNA sequencing (RAD-Seq) data. Although summarization is an essential step in RAD-Seq analysis, no specific tools exist for summarizing and visualizing these data. Stacksbinder generates a summary report by using files exported from Stacks. The report is HTML-based and easy to browse in any environment. It consists of plots and tables containing information that can be used to assess the quality of experiments and determine the filtering of single nucleotide polymorphisms.

Stacksbinder（可从https://ps.agr.ryukoku.ac.jp/stacksbinder/获得）是一种基于Web的工具，用于汇总软件Stacks的输出，该工具广泛用于分析与限制性酶切位点相关的DNA测序（RAD- Seq）数据。尽管汇总是RAD-Seq分析中必不可少的步骤，但是不存在用于汇总和可视化这些数据的特定工具。Stacksbinder使用从Stacks导出的文件生成摘要报告。该报告基于HTML，可在任何环境中轻松浏览。它由包含可用于评估实验质量并确定单核苷酸多态性过滤信息的图表组成。