We describe novel hybrid primers for the successful amplification and sequencing of the mitochondrial cytochrome b (cytb) gene in the family Siganidae. We aligned 403 cytb sequences from 29 species and found multiple potential conserved hybrid primers (< twofold degeneracy). The best set was selected, and successfully amplified and sequenced ~ 1100 bp of cytb in the Siganidae.

中文翻译：

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新型杂交引物，用于家兔鱼线粒体细胞色素b基因的测序（Teleostei：Siganidae）

我们描述了新型杂交引物，用于成功扩增和测序线虫科中的线粒体细胞色素b（cyt b）基因。我们比对了来自29种的403个cyt b序列，发现了多个潜在的保守杂交引物（<双重简并）。选择最佳组，并成功地扩增并测序了丝虫科中cyt b的〜1100 bp 。